AB161136

Recombinant Human MASP2 protein

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Recombinant Human MASP2 protein is a Human Full Length protein, in the 1 to 686 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

Mannan-binding lectin serine protease 2, MBL-associated serine protease 2, Mannose-binding protein-associated serine protease 2, MASP-2, MASP2

1 Images

SDS-PAGE - Recombinant Human MASP2 protein (AB161136)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MASP2 protein (AB161136)

ab161136 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

発現系

Wheat germ

アプリケーション

WB, ELISA, SDS-PAGE

applications

生物活性

アクセッション番号

O00187

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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配列情報

[{"sequence":"MRLLTLLGLLCGSVATPLGPKWPEPVFGRLASPGFPGEYANDQERRWTLTAPPGYRLRLYFTHFDLELSHLCEYDFVKLSSGAKVLATLCGQESTDTERAPGKDTFYSLGSSLDITFRSDYSNEKPFTGFEAFYAAEDIDECQVAPGEAPTCDHHCHNHLGGFYCSCRAGYVLHRNKRTCSALCSGQVFTQRSGELSSPEYPRPYPKLSSCTYSISLEEGFSVILDFVESFDVETHPETLCPYDFLKIQTDREEHGPFCGKTLPHRIETKSNTVTITFVTDESGDHTGWKIHYTSTAQPCPYPMAPPNGHVSPVQAKYILKDSFSIFCETGYELLQGHLPLKSFTAVCQKDGSWDRPMPACSIVDCGPPDDLPSGRVEYITGPGVTTYKAVIQYSCEETFYTMKVNDGKYVCEADGFWTSSKGEKSLPVCEPVCGLSARTTGGRIYGGQKAKPGDFPWQVLILGGTTAAGALLYDNWVLTAAHAVYEQKHDASALDIRMGTLKRLSPHYTQAWSEAVFIHEGYTHDAGFDNDIALIKLNNKVVINSNITPICLPRKEAESFMRTDDIGTASGWGLTQRGFLARNLMYVDIPIVDHQKCTAAYEKPPYPRGSVTANMLCAGLESGGKDSCRGDSGGALVFLDSETERWFVGGIVSWGSMNCGEAGQYGVYTKVINYIPWIENIISDF","proteinLength":"Full Length","predictedMolecularWeight":"102 kDa","actualMolecularWeight":null,"aminoAcidEnd":686,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O00187","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MASP-2 also known as Mannan-binding lectin serine protease 2 is an important component of the innate immune system. This protein has a molecular mass of approximately 76 kDa and is expressed in the liver before being released into the bloodstream. MASP-2 primarily acts during the lectin pathway of the complement system where it plays an essential role in immune defense. The protein activates the complement cascade upon binding with carbohydrate molecules on pathogen surfaces contributing to opsonization and direct lysis of pathogens.

Biological function summary

MASP-2 associates with MBL (Mannose-binding lectin) and forms a complex critical for the lectin pathway's function. In this complex MASP-2 initiates the cleavage of complement proteins C4 and C2 forming the C3 convertase. This action enhances the immune response by promoting the clearance of pathogens. MASP-1 is another protease that works closely with MASP-2 in this pathway although the two have distinct roles—MASP-2 is primarily responsible for the activation of the downstream components of the cascade.

Pathways

MASP-2 plays an integral role in the complement system and particularly in the lectin pathway which is one of the three pathways of complement activation. It connects with both the classical and alternative pathways through the formation of C3 convertase a critical step in the complement cascade. The interactions with other proteins like MASP-1 and MBL are significant; MASP-1 has an additional role in the activation sequence by further augmenting MASP-2's activity and facilitating efficient pathogen clearance.

MASP-2 is connected to conditions like autoimmune diseases and recurrent infections due to its involvement in the complement system. For instance MASP-2 deficiency can lead to increased susceptibility to infections reflecting its role in pathogen clearance. The protein has been studied in relation to systemic lupus erythematosus where dysregulation of the complement pathway can exacerbate disease symptoms. Proteins such as MBL and MASP-1 which interact closely with MASP-2 also have roles in these disease states and further illustrate the importance of proper complement system functioning in disease prevention and control.

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製品の性状

製品の状態

Liquid

補足情報

Glutathione Sepharose 4 Fast Flow

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一般的な情報

機能

Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.

配列の類似性

Belongs to the peptidase S1 family.

翻訳後修飾

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.. Activated by cleavage after Arg-444. The uncleaved zymogen is inactive towards synthetic substrates, but has sufficient activity to effect autocatalytic cleavage.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information MASP2

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Abcam product promise

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Recombinant Human MASP2 protein