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AB105211

Recombinant human MARK4 protein

Recombinant human MARK4 protein

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(1 Publication)

Recombinant human MARK4 protein is a Human Full Length protein, in the 1 to 752 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

KIAA1860, MARKL1, MARK4, MAP/microtubule affinity-regulating kinase 4, MAP/microtubule affinity-regulating kinase-like 1

4 Images
Functional Studies - Recombinant human MARK4 protein (AB105211)
  • FuncS

Unknown

Functional Studies - Recombinant human MARK4 protein (AB105211)

The Specific activity of ab105211 was determined to be 1050 nmol/min/mg.

Functional Studies - Recombinant human MARK4 protein (AB105211)
  • FuncS

Unknown

Functional Studies - Recombinant human MARK4 protein (AB105211)

The specific activity of MARK4 (ab105211) was determined to be 1140 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human MARK4 protein (AB105211)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MARK4 protein (AB105211)

SDS-PAGE showing ab105211 at approximately 104kDa.

SDS-PAGE - Recombinant human MARK4 protein (AB105211)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human MARK4 protein (AB105211)

SDS PAGE analysis of ab105211

Key facts

精製度

>90% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, WB, FuncS

applications

生物活性

Yes

生物学的活性

The Specific activity of ab105211 was determined to be 1050 nmol/min/mg.

アクセッション番号

Q96L34

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

ab204854 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"104 kDa","actualMolecularWeight":null,"aminoAcidEnd":752,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q96L34","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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製品の性状

製品の状態

Liquid

補足情報

Purity was determined to be >90% by densitometry. Affinity purified.

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一般的な情報

機能

Serine/threonine-protein kinase (PubMed : 14594945, PubMed : 15009667, PubMed : 23184942, PubMed : 23666762). Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 14594945, PubMed : 23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed : 14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed : 14594945, PubMed : 25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed : 23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed : 23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed : 25123532). Reduces neuronal cell survival (PubMed : 15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed : 23184942). Involved in NLRP3 positioning along microtubules by mediating NLRP3 recruitment to microtubule organizing center (MTOC) upon inflammasome activation (PubMed : 28656979).

配列の類似性

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

翻訳後修飾

Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.. Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39 (PubMed:14976552). Phosphorylated throughout the cell cycle (PubMed:25123532).

細胞内局在性

Cytoskeleton

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製品プロトコール

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ターゲットの情報

Serine/threonine-protein kinase (PubMed : 14594945, PubMed : 15009667, PubMed : 23184942, PubMed : 23666762). Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 14594945, PubMed : 23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed : 14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed : 14594945, PubMed : 25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed : 23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed : 23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed : 25123532). Reduces neuronal cell survival (PubMed : 15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed : 23184942). Involved in NLRP3 positioning along microtubules by mediating NLRP3 recruitment to microtubule organizing center (MTOC) upon inflammasome activation (PubMed : 28656979).
See full target information MARK4
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 7:135 PubMed31429800

2019

Ischemic axonal injury up-regulates MARK4 in cortical neurons and primes tau phosphorylation and aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Y Hayden,Jennifer Putman,Stefanie Nunez,Woo Shik Shin,Mandavi Oberoi,Malena Charreton,Suman Dutta,Zizheng Li,Yutaro Komuro,Mary Teena Joy,Gal Bitan,Allan MacKenzie-Graham,Lin Jiang,Jason D Hinman
View all publications
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