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AB174389

Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus)

Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus)

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Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus) is a Human Full Length protein, in the 20 to 335 aa range, expressed in Escherichia coli, with >85%, suitable for Mass Spec, SDS-PAGE.

別名を表示する

MAP1D, METAP1D, MAP 1D, MetAP 1D, Peptidase M 1D

1 Images
SDS-PAGE - Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus) (AB174389)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human MAP1D protein - BSA and Azide free (His tag N-Terminus) (AB174389)

SDS-PAGE analysis MAP1D using 3 μg of ab174389 on a 15% gel.

Key facts

精製度

>85% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

No

アクセッション番号

Q6UB28

アニマルフリー

No

キャリアフリー

Yes

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris-HCl buffer, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at 3 μg/lane</p>" } } }

配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSSPLNHIYLHKQSSSQQRRNFFFRRQRDISHSIVLPAAVSSAHPVPKHIKKPDYVTTGIVPDWGDSIEVKNEDQIQGLHQACQLARHVLLLAGKSLKVDMTTEEIDALVHREIISHNAYPSPLGYGGFPKSVCTSVNNVLCHGIPDSRPLQDGDIINIDVTVYYNGYHGDTSETFLVGNVDECGKKLVEVARRCRDEAIAACRAGAPFSVIGNTISHITHQNGFQVCPHFVGHGIGSYFHGHPEIWHHANDSDLPMEEGMAFTIEPIITEGSPEFKVLEDAWTVVSLDNQRSAQFEHTVLITSRGAQILTKLPHEA","proteinLength":"Full Length","predictedMolecularWeight":"37.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":335,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6UB28","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False

製品の性状

製品の状態

Liquid

補足情報

ab174389 is purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20 mM Tris pH 7.5, 2 mM EDTA.

一般的な情報

機能

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis.

配列の類似性

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.

細胞内局在性

Mitochondrion

製品プロトコール

ターゲットの情報

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed (By similarity). May play a role in colon tumorigenesis.
See full target information METAP1D

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