AB114358

Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus)

Name: Recombinant Human MADH7/SMAD7 protein (ab114358) | Abcam
Brand: Abcam Limited
SKU: AB114358
Price: 125000 JPY
Availability: InStock

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Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus) is a Human Fragment protein, in the 160 to 260 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

MADH7, MADH8, SMAD7, SMAD family member 7, SMAD 7, hSMAD7, Mothers against decapentaplegic homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 7, Mothers against DPP homolog 7, MAD homolog 8, Mothers against DPP homolog 8

1 Images

SDS-PAGE - Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus) (AB114358)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus) (AB114358)

12.5% SDS-PAGE showing ab114358 at approximately 36.74kDa stained with Coomassie Blue.

Key facts

発現系

Wheat germ

アプリケーション

WB, SDS-PAGE, ELISA

applications

生物活性

アクセッション番号

O15105

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"(Recombinant protein)." } } }

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配列情報

[{"sequence":"CKVFRWPDLRHSSEVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPTADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSH","proteinLength":"Fragment","predictedMolecularWeight":"36.74 kDa","actualMolecularWeight":null,"aminoAcidEnd":260,"aminoAcidStart":160,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O15105","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MADH7 also known as SMAD7 is an intracellular protein that acts as an inhibitor in the signaling pathway of the TGF-beta (transforming growth factor-beta) family. This protein has a molecular mass of approximately 46 kDa and is widely expressed with a significant presence in tissues involved in immune response and development. SMAD7 directly interacts with receptor-regulated SMADs (R-SMADs) preventing their phosphorylation and consequent nuclear translocation effectively halting further signal transduction.

Biological function summary

SMAD7 functions to regulate the TGF-beta signaling pathway by operating as a negative feedback mediator. It binds to TGF-beta receptors recruiting E3 ubiquitin ligases which promote receptor degradation. SMAD7 is not part of complex large-scale multi-protein assemblies but is essential in modulating the pathway's activity. Its balance controls important processes like cell proliferation differentiation and apoptosis across varied biological contexts.

Pathways

SMAD7 strongly influences the TGF-beta and BMP (bone morphogenetic protein) signaling pathways. SMAD7 blocks TGF-beta signaling by interfering with the activity of R-SMADs such as SMAD2 and SMAD3 and it modulates the BMP pathway by interacting with SMAD1 and SMAD5. Its regulatory roles are tightly integrated within these pathways highlighting its importance in cellular homeostasis and response to extracellular signals.

SMAD7 has links to inflammatory conditions and cancer. Its dysregulation can lead to heightened TGF-beta signaling which may contribute to conditions like fibrosis and can enhance tumor progression by affecting cancer cell dynamics and the tumor microenvironment. In these scenarios abnormal SMAD7 function can associate with proteins such as SMAD3 in fibrosis while in cancer its association with proteins like SMAD4 disrupts normal growth control and cellular response.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access (PubMed : 21791611). Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

配列の類似性

Belongs to the dwarfin/SMAD family.

翻訳後修飾

Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.. Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation (PubMed:14657019, PubMed:16601693). In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation (PubMed:11278251).. Acetylation prevents ubiquitination and degradation mediated by SMURF1.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information SMAD7

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Abcam product promise

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Recombinant Human MADH7/SMAD7 protein (GST tag N-Terminus)