AB62015

Recombinant human M-CSF protein (Active)

Bioactive
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Recombinant human M-CSF protein (Active) is a Human Fragment protein, in the 33 to 190 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for FuncS, SDS-PAGE.

別名を表示する

Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Proteoglycan macrophage colony-stimulating factor, PG-M-CSF, CSF1

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Functional Studies - Recombinant human M-CSF protein (Active) (AB62015)

FuncS

Supplier Data

Functional Studies - Recombinant human M-CSF protein (Active) (AB62015)

ab62015 Induced Proliferation of NFS-60 Cells.

SDS-PAGE - Recombinant human M-CSF protein (Active) (AB62015)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human M-CSF protein (Active) (AB62015)

1 ug of ab62015 on 4-20% Tris-Glycerine SDS-PAGE, stained with Coomassie Blue.

Lane 1 : Non reducing

Lane 2 : Reducing

Key facts

精製度

>95% SDS-PAGE

エンドトキシンレベル

<1 EU/µg

発現系

Escherichia coli

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Method of Determination: NFS-60 cell proliferation

Acceptance Criteria: ED₅₀ ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)

The specific activity of Human M-CSF is 1.0 x 10^5 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard.

アクセッション番号

P09603

アニマルフリー

キャリアフリー

Yes

種

Human

再構成

Reconstitute at 0.1 mg/mL in water

バッファー組成

Constituents: 0.58% Sodium chloride, 0.16% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Dimer: 37.1 kDa</p>" } } }

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配列情報

[{"sequence":"MEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQGHERQSEGSMEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQGHERQSEGS","proteinLength":"Fragment","predictedMolecularWeight":"37.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":190,"aminoAcidStart":33,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09603","tags":[]}]

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon reconstitution add a carrier protein (0.1% BSA)

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

M-CSF or macrophage colony-stimulating factor is a cytokine involved in the regulation of macrophage production. Alternative names for M-CSF include CSF-1 and colony-stimulating factor 1. The M-CSF protein has a molecular mass of approximately 18 to 22 kDa. M-CSF is mainly expressed in various tissues including the placenta kidney and bone marrow. It functions as a homodimer important for the survival proliferation and differentiation of mononuclear phagocyte lineage cells.

Biological function summary

M-CSF influences the production differentiation and function of macrophages and osteoclasts. It is not part of a large complex but interacts with its receptor the CSF1R (colony-stimulating factor 1 receptor) on the surface of target cells. This interaction promotes the survival and proliferation of the target cells playing significant roles in immune response and bone homeostasis by regulating osteoclast development.

Pathways

The interaction of M-CSF with its receptor is central to several biological pathways notably the immune system pathway and bone resorption pathway. Within these pathways the binding of M-CSF to CSF1R activates downstream signaling cascades such as the PI3K/AKT and MAPK pathways importantly affecting cell survival and differentiation. The M-CSF pathway intersects with other cytokines and factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which also regulate immune cell dynamics.

Dysregulation of M-CSF levels is implicated in conditions such as osteoporosis and certain cancers. In osteoporosis M-CSF’s role in osteoclast development links to increased bone resorption leading to bone loss. In cancers M-CSF overexpression may facilitate tumor-associated macrophage infiltration therefore supporting tumor progression. The interaction with CSF1R is significant as it serves as a potential target for therapeutic strategies aimed at modulating macrophage activity in these diseases.

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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.

翻訳後修飾

N-glycosylated.. Isoform 1. N-glycosylated.. Isoform 3. N-glycosylated.. O-glycosylated; contains chondroitin sulfate (PubMed:1531650, PubMed:8051056). O-glycosylated with core 1 or possibly core 8 glycans (PubMed:22171320, PubMed:23234360, PubMed:3264877).. Isoform 1. O-glycosylated.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information CSF1

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Recombinant human M-CSF protein (Active)