Recombinant Human LysRS protein (ab113142)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant Human LysRS protein -
精製度
> 95 % SDS-PAGE.
ab113142 was purified using conventional chromatography. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
MGSSHHHHHHSSGLVPRGSHMGSGVGPEEESVDPNQYYKIRSQAIHQLKV NGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASG GKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPG KTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDF VRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNE LDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCE FYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTP PFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRT TARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELF VMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEY GLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLE STTVGTSV -
予測される分子量
64 kDa including tags -
領域
63 to 597 -
タグ
His tag N-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab113142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Liquid -
備考
Previously labelled as Lysyl tRNA synthetase.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.02% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
関連情報
-
別名
- CMTRIB
- DFNB89
- EC 6.1.1.6
see all -
機能
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. -
関連疾患
Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. -
配列類似性
Belongs to the class-II aminoacyl-tRNA synthetase family. -
ドメイン
The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2. -
細胞内局在
Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha. - Information by UniProt
画像
-
SDS-PAGE - Recombinant Human LysRS protein (ab113142)15% SDS-PAGE analysis of ab113142 (3µg)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab113142 は論文での使用が確認できていません。