Recombinant Human Lipoprotein lipase (ab115504)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 80% Densitometry
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human Lipoprotein lipase
Lipoprotein lipase タンパク質・ペプチド 製品一覧 -
精製度
> 80 % Densitometry.
ab115504 is filtered (0.4 µm). -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
HVDYKDDDDKPAGADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESV ATCHFNHSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWL SRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHA AGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTR GSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLV KCSHERSIHLFIDSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLG YEISKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTESETHTNQAFEI SLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSY FSWSDWWSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVK CHDKSLNKKSG -
予測される分子量
52 kDa including tags -
領域
28 to 475 -
タグ
DDDDK tag N-Terminus
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製品の詳細
Recombinant Human Lipoprotein lipase
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab115504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C.
pH: 7.50
Constituents: 0.24% Tris buffer, 0.29% Sodium chloride -
再構成Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
関連情報
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別名
- EC 3.1.1
- EC 3.1.1.34
- HDLCQ11
see all -
機能
The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. -
関連疾患
Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency) [MIM:238600]; also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. -
配列類似性
Belongs to the AB hydrolase superfamily. Lipase family.
Contains 1 PLAT domain. -
翻訳後修飾
Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. -
細胞内局在
Cell membrane. Secreted. Locates to the plasma membrane of microvilli of hepatocytes with triacyl-glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human Lipoprotein lipase (ab115504)14% SDS-PAGE showing ab115504
Lane 1: M.W. marker – 14, 21, 31, 45, 66, 97 kDa
Lane 2: reduced and boiled sample, 5μg/lane.
Lane 3: non-reduced and non-boiled sample, 5μg/lane.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab115504 は論文での使用が確認できていません。
