JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB115504

Recombinant Human Lipoprotein lipase

Recombinant Human Lipoprotein lipase

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Lipoprotein lipase is a Human Full Length protein, in the 28 to 475 aa range, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, WB.

別名を表示する

LIPD, LPL, Lipoprotein lipase, Phospholipase A1

1 Images
SDS-PAGE - Recombinant Human Lipoprotein lipase (AB115504)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Lipoprotein lipase (AB115504)

14% SDS-PAGE showing ab115504
Lane 1 : M.W. marker – 14, 21, 31, 45, 66, 97 kDa
Lane 2 : reduced and boiled sample, 5μg/lane.
Lane 3 : non-reduced and non-boiled sample, 5μg/lane.

Key facts

精製度

>80% Densitometry

発現系

HEK 293 cells

タグ

DDDDK tag N-Terminus

アプリケーション

WB, SDS-PAGE

applications

生物活性

No

アクセッション番号

P06858

アニマルフリー

No

キャリアフリー

No

種

Human

再構成

Reconstitute at 0.5 mg/mL in water

バッファー組成

pH: 7.5 Constituents: 0.29% Sodium chloride, 0.24% Tris buffer

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

配列情報

[{"sequence":"HVDYKDDDDKPAGADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESVATCHFNHSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLSRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHAAGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERSIHLFIDSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLGYEISKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTESETHTNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSYFSWSDWWSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVKCHDKSLNKKSG","proteinLength":"Full Length","predictedMolecularWeight":"51.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":475,"aminoAcidStart":28,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P06858","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]}]
style
left-column

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
-80°C
長期保存温度
-80°C
False
style
left-column
style
left-column

製品の性状

製品の状態

Lyophilized

補足情報

ab115504 is filtered (0.4 µm).

style
left-column

一般的な情報

機能

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).

配列の類似性

Belongs to the AB hydrolase superfamily. Lipase family.

翻訳後修飾

Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.

style
left-column

製品プロトコール

style
left-column

ターゲットの情報

Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage (PubMed : 11342582, PubMed : 27578112, PubMed : 8675619). Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity (PubMed : 12032167, PubMed : 7592706). Mediates margination of triglyceride-rich lipoprotein particles in capillaries (PubMed : 24726386). Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans (PubMed : 11342582, PubMed : 27811232).
See full target information LPL
style
left-column
style
left-column
style
right-column

組合せ製品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

  • 0
  • 0
Assay Kits

AB204721

Lipoprotein Lipase Assay Kit (Fluorometric)

Functional Studies - Lipoprotein Lipase Assay Kit (Fluorometric) (AB204721)

  • 0
  • 0
Primary Antibodies

AB21356

Anti-Lipoprotein lipase antibody [LPL.A4]

Flow Cytometry - Anti-Lipoprotein lipase antibody [LPL.A4] (AB21356)

  • 4
  • 8
Assay Kits

AB102524

Lipase Assay Kit (Colorimetric)

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)

  • 0
  • 0
Proteins & Peptides

AB226233

Recombinant Human Adipose Triglyceride Lipase protein (His tag)

SDS-PAGE - Recombinant Human Adipose Triglyceride Lipase protein (His tag) (AB226233)

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com