Recombinant Human Lipoamide Dehydrogenase protein (ab93679)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, WB
製品の詳細
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製品名
Recombinant Human Lipoamide Dehydrogenase protein -
生理活性
Specific activity is > 200 units/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C.
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精製度
> 95 % SDS-PAGE.
ab93679 is purified using conventional chromatography techniques. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab93679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Dehydrogenase complex, E3 component
- Diaphorase
- Dihydrolipoamide dehydrogenase
see all -
機能
Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. -
関連疾患
Note=Defects in DLD are involved in the development of congenital infantile lactic acidosis.
Defects in DLD are a cause of maple syrup urine disease (MSUD) [MIM:248600]. MSUD is characterized by mental and physical retardation, feeding problems and a maple syrup odor to the urine. The keto acids of the branched-chain amino acids are present in the urine, resulting from a block in oxidative decarboxylation. -
配列類似性
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. -
翻訳後修飾
Tyrosine phosphorylated. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human Lipoamide Dehydrogenase protein (ab93679)
3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
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Western blot - Recombinant Human Lipoamide Dehydrogenase protein (ab93679)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab93679 は論文での使用が確認できていません。