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AB271600

Recombinant Human Lin28B protein (DDDDK tag N-Terminus + Avi tag N-Terminus)

Recombinant Human Lin28B protein (DDDDK tag N-Terminus + Avi tag N-Terminus)

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Recombinant Human Lin28B protein (DDDDK tag N-Terminus + Avi tag N-Terminus) is a Human Full Length protein, in the 2 to 250 aa range, expressed in HEK 293 cells, with >70%, suitable for SDS-PAGE.

別名を表示する

CSDD2, LIN28B, Protein lin-28 homolog B, Lin-28B

1 Images
SDS-PAGE - Recombinant Human Lin28B protein (DDDDK tag N-Terminus + Avi tag N-Terminus) (AB271600)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Lin28B protein (DDDDK tag N-Terminus + Avi tag N-Terminus) (AB271600)

SDS-PAGE analysis of 2 μg ab271600.

Key facts

精製度

>70% SDS-PAGE

発現系

HEK 293 cells

タグ

DDDDK tag N-Terminus Avi tag N-Terminus

アプリケーション

SDS-PAGE

applications

生物活性

No

アクセッション番号

Q6ZN17

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"AEGGASKGGGEEPGKLPEPAEEESQVLRGTGHCKWFNVRMGFGFISMINREGSPLDIPVDVFVHQSKLFMEGFRSLKEGEPVEFTFKKSSKGLESIRVTGPGGSPCLGSERRPKGKTLQKRKPKGDRCYNCGGLDHHAKECSLPPQPKKCHYCQSIMHMVANCPHKNVAQPPASSQGRQEAESQPCTSTLPREVGGGHGCTSPPFPQEARAEISERSGRSPQEASSTKSSIAPEEQSKKGPSVQKRKKT","proteinLength":"Full Length","predictedMolecularWeight":"30 kDa","actualMolecularWeight":null,"aminoAcidEnd":250,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q6ZN17","tags":[{"tag":"DDDDK","terminus":"N-Terminus"},{"tag":"Avi","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
保管に関する情報
Avoid freeze / thaw cycle
False
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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed : 22118463). Does not act on pri-miR21 (PubMed : 22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed : 19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed : 22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

配列の類似性

Belongs to the lin-28 family.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA (PubMed : 22118463). Does not act on pri-miR21 (PubMed : 22118463). The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation (PubMed : 19703396). This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent (PubMed : 22118463). Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). When overexpressed, isoform 1 stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
See full target information LIN28B
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組合せ製品

Primary Antibodies

AB191881

Anti-Lin28B antibody [EPR18717]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Lin28B antibody [EPR18717] (AB191881)

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Proteins & Peptides

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Proteins & Peptides

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Recombinant human Yes1 protein (GST tag N-Terminus)

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Proteins & Peptides

AB198661

Recombinant Human WTAP protein

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