AB123215

Recombinant Human LASP1 protein (His tag N-Terminus)

Name: Recombinant Human LASP1 protein (ab123215) | Abcam
Brand: Abcam Limited
SKU: AB123215
Price: 88000 JPY
Availability: InStock

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Recombinant Human LASP1 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 261 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

MLN50, LASP1, LIM and SH3 domain protein 1, LASP-1, Metastatic lymph node gene 50 protein, MLN 50

1 Images

SDS-PAGE - Recombinant Human LASP1 protein (His tag N-Terminus) (AB123215)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human LASP1 protein (His tag N-Terminus) (AB123215)

15% SDS-PAGE analysis of ab123215 (3 μg).

Key facts

精製度

>80% SDS-PAGE

発現系

Escherichia coli

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

アクセッション番号

Q14847

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMNPNCARCGKIVYPTEKVNCLDKFWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTMVADTPENLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHEEFEKSRMGPSGGEGMEPERRDSQDGSSYRRPLEQQQPHHIPTSAPVYQQPQQQPVAQSYGGYKEPAAPVSIQRSAPGGGGKRYRAVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGTVERTGDTGMLPANYVEAI","proteinLength":"Full Length","predictedMolecularWeight":"32.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":261,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q14847","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The LASP1 protein also known as LIM and SH3 protein 1 is a 261 amino acid protein with an approximate mass of 29 kDa. It acts as an actin-binding protein involved in the dynamics of the cytoskeleton. LASP1 is expressed in various tissues with higher expression seen in the kidneys lungs and breast tissues. Its structure includes an LIM domain at the N-terminal and an SH3 domain at the C-terminal essential for interactions with other proteins and cytoskeletal components.

Biological function summary

LASP1 plays a significant role in cell migration and adhesion processes. It associates with cytoskeletal proteins such as F-actin helping in the stabilization and organization of the cytoskeleton. LASP1 forms part of complexes with zyxin and vasodilator-stimulated phosphoprotein (VASP) at focal adhesions contributing to cellular motility and signaling. It influences processes like cytokinesis and wound healing which rely on precise cytoskeletal rearrangements.

Pathways

Researchers have identified LASP1 as being involved in the ERK/MAPK and PI3K/AKT signaling pathways. It links to these pathways through interactions promoting cell proliferation and survival. This protein associates with other pathway-related proteins like Grb2 and Src which play roles in transmitting signals from cell surface receptors to the nucleus impacting gene expression and cell cycle progression.

LASP1 overexpression often associates with various cancers particularly breast cancer and colorectal cancer. In these contexts LASP1 contributes to tumor progression and metastasis. Its interaction with proteins such as EGFR and HER2 has widespread implications in oncogenic signaling. Understanding LASP1's role in disease can enhance diagnostic and therapeutic strategies targeting these malignancies.

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製品の性状

製品の状態

Liquid

補足情報

ab123215 is purified using conventional chromatography techniques. It is >80% pure by SDS-PAGE.

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一般的な情報

機能

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).

細胞内局在性

Cytoskeleton

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information LASP1

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Abcam product promise

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Recombinant Human LASP1 protein (His tag N-Terminus)