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AB316534

Recombinant Human LAMP2 Protein Standard

Recombinant Human LAMP2 Protein Standard

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Recombinant Human LAMP2 Protein Standard is a Human Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.

別名を表示する

CD107b, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, LAMP2

2 Images
Sandwich ELISA - Recombinant Human LAMP2 Protein Standard (AB316534)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human LAMP2 Protein Standard (AB316534)

Sandwich ELISA with the capture antibody ab288606 dilution at 2 μg/mL and detector antibody ab288624 dilution at 0.5 μg/mL after biotin conjugation.

SDS-PAGE - Recombinant Human LAMP2 Protein Standard (AB316534)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human LAMP2 Protein Standard (AB316534)

SDS-PAGE analysis of ab316534 under reducing conditions for 2ug protein.

Key facts

精製度

>80% SDS-PAGE

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

SDS-PAGE, sELISA

applications

生物活性

No

アクセッション番号

P13473

アニマルフリー

Yes

キャリアフリー

No

種

Human

バッファー組成

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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組合せ製品

Primary Antibodies

AB125068

Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

20ul selling size|RabMAb|Recombinant|Lab Essentials|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB125068)

  • 4
  • 4
Secondary Antibodies

AB205720

Goat Anti-Rat IgG H&L (HRP)

Western blot - Goat Anti-Rat IgG H&L (HRP) (AB205720)

  • 5
  • 1
Assay Kits

AB313912

Human Niemann Pick C1 ELISA Kit

Sandwich ELISA - Human Niemann Pick C1 ELISA Kit (AB313912)

  • 0
  • 0
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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配列情報

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"38 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P13473","tags":[]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification His Tag
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

LAMP2 also known as CD107b is an important protein found in the lysosomal membrane. It plays a significant role in lysosome function facilitating autophagy and the degradation of cellular debris. LAMP2 proteins are expressed in most tissues especially in tissues with high lysosomal activity like the liver kidney and heart. The LAMP2 molecule has a molecular weight of approximately 45 kDa. Researchers frequently use LAMP2 staining and LAMP2 marker to study its cellular localization and expression levels.
Biological function summary

LAMP2 is essential for the normal fusion of lysosomes with autophagosomes. This process ensures the recycling of cellular components and maintenance of cellular homeostasis. LAMP2 acts in conjunction with other members of the lysosome-associated membrane protein family LAMP1 and LAMP3 forming part of a larger complex. This interaction is fundamental in managing the degradation pathways within the cell supporting processes such as lipid metabolism and protein turnover.

Pathways

The activity of LAMP2 is critical in the autophagic and degradation pathways within the cell. It particularly interacts in the autophagy-lysosome pathway where it aids the clearance of damaged organelles and protein aggregates. LAMP2 interaction with proteins such as the autophagy-related protein LC3 helps this degradation process. Additionally LAMP2 plays a role in endocytic pathways linking it to proteins involved in vesicle trafficking and membrane fusion.

Mutations or deficiencies in LAMP2 are associated with Danon disease a type of lysosomal storage disorder. Patients with Danon disease exhibit symptoms such as cardiomyopathy skeletal myopathy and intellectual disabilities. LAMP2 is also implicated in other lysosomal disorders with a connection to the malfunction of autophagy processes. Its dysfunction is often linked with proteins responsible for cellular degradation affecting the normal homeostatic balance and leading to the accumulation of autophagic substrates.
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一般的な情報

機能

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 27628032, PubMed : 36586411, PubMed : 37390818, PubMed : 8662539). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed : 37390818). Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 27628032, PubMed : 36586411, PubMed : 8662539). Functions by binding target proteins, such as GAPDH, GPX4, NLRP3 and MLLT11, and targeting them for lysosomal degradation (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 36586411, PubMed : 8662539). In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2 (PubMed : 36586411). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed : 27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed : 27628032). Required for normal degradation of the contents of autophagosomes (PubMed : 27628032). Required for efficient MHC class II-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHC II subunits (PubMed : 15894275, PubMed : 20518820). Is not required for efficient MHC class II-mediated presentation of endogenous antigens (PubMed : 20518820).. Isoform LAMP-2C. Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

配列の類似性

Belongs to the LAMP family.

翻訳後修飾

O- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans.

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製品プロトコール

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ターゲットの情報

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 27628032, PubMed : 36586411, PubMed : 37390818, PubMed : 8662539). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed : 37390818). Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 27628032, PubMed : 36586411, PubMed : 8662539). Functions by binding target proteins, such as GAPDH, GPX4, NLRP3 and MLLT11, and targeting them for lysosomal degradation (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 36586411, PubMed : 8662539). In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2 (PubMed : 36586411). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed : 27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed : 27628032). Required for normal degradation of the contents of autophagosomes (PubMed : 27628032). Required for efficient MHC class II-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHC II subunits (PubMed : 15894275, PubMed : 20518820). Is not required for efficient MHC class II-mediated presentation of endogenous antigens (PubMed : 20518820).. Isoform LAMP-2C. Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
See full target information LAMP2
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