AB156968

Recombinant Human KRAS protein

Name: Recombinant Human KRAS protein (ab156968) | Abcam
Brand: Abcam Limited
SKU: AB156968
Price: 78000 JPY
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(2 Publications)

Recombinant Human KRAS protein is a Human Full Length protein, in the 1 to 186 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

KRAS2, RASK2, KRAS, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

1 Images

SDS-PAGE - Recombinant Human KRAS protein (AB156968)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human KRAS protein (AB156968)

15% SDS-PAGE analysis of ab156968 (3 µg).

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

アクセッション番号

P01116

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Isoform 2A. The mass of this protein was confirmed by mass spectroscopy.

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTPGCVKIKKC","proteinLength":"Full Length","predictedMolecularWeight":"23.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":186,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01116","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The KRAS protein also known as Kirsten rat sarcoma viral oncogene homolog is a small GTPase weighing around 21 kDa. It plays an important role in cell signaling pathways and is widely expressed in various tissues. As a molecular switch KRAS cycles between an active GTP-bound state and an inactive GDP-bound state regulating cell proliferation differentiation and survival. KRAS mutations are often studied using various cell lines including the HCT116 line which is a human colorectal carcinoma cell line with a KRAS mutant background. Western blot analysis commonly helps in investigating KRAS protein expression and activity levels.

Biological function summary

KRAS influences many cellular processes. It is not part of a complex but acts alone within its pathways. It modulates signal transduction processes which are significant for the control of proliferation apoptosis and cell cycle progress. Mutations within this protein such as KRAS G12V and KRAS G12D lead to continuous activation and can cause uncontrolled cell growth which links them to various cancers. KRAS commonly interacts with proteins like RAF kinase and PI3K signaling components to execute its functions.

Pathways

KRAS is an important component in at least two important signaling pathways: the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways play major roles in regulating gene expression that drives cell growth and division. In the MAPK/ERK pathway KRAS activates RAF which subsequently initiates a phosphorylation cascade that stimulates ERK. This protein also interacts with elements of the PI3K/AKT pathway influencing cellular survival and metabolism. These interactions position KRAS as a vital oncogene in cancer biology.

KRAS mutations are important in the development and progression of colorectal and pancreatic cancers. The persistence of active KRAS leads to continuous signaling that drives tumor growth and resistance to apoptosis. KRAS-associated cancers often show poor prognosis partly due to limited therapeutic options. Its interaction with proteins like EGFR (Epidermal Growth Factor Receptor) in various cancers highlights complex treatment challenges as these proteins can provide alternative signaling routes further complicating disease management.

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製品の性状

製品の状態

Liquid

補足情報

ab156968 purified using conventional chromatography techniques.

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一般的な情報

機能

The protein expressed by the KRAS gene binds GDP/GTP and has intrinsic GTPase activity. It plays an important role in regulating cell proliferation and may contribute to oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells through a ZNF304-dependent mechanism. This supplementary information is collated from multiple sources and compiled automatically.

配列の類似性

Belongs to the small GTPase superfamily. Ras family.

翻訳後修飾

Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).. Palmitoylated at Lys-182, Lys-184 and Lys-185 (PubMed:29239724). Palmitoylation on lysine residues is promoted by palmitoylation at Cys-180 (PubMed:29239724). Lysine-depalmitoylation by SIRT2 promotes its localization to endomembranes in endocytic pathways (PubMed:29239724).. Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.. (Microbial infection) Glucosylated at Thr-35 by P.sordellii toxin TcsL.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information KRAS

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 25:159-169 PubMed36635501

2023

Multiplexed screens identify RAS paralogues HRAS and NRAS as suppressors of KRAS-driven lung cancer growth.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Tang,Emily G Shuldiner,Marcus Kelly,Christopher W Murray,Jess D Hebert,Laura Andrejka,Min K Tsai,Nicholas W Hughes,Mitchell I Parker,Hongchen Cai,Yao-Cheng Li,Geoffrey M Wahl,Roland L Dunbrack,Peter K Jackson,Dmitri A Petrov,Monte M Winslow

Scientific reports 10:21671 PubMed33303890

2020

Generation of KS-58 as the first K-Ras(G12D)-inhibitory peptide presenting anti-cancer activity in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Kotaro Sakamoto,Teruaki Masutani,Takatsugu Hirokawa

View all publications

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Abcam product promise

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Recombinant Human KRAS protein