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AB323152

Recombinant Human KMT4/Dot1L Protein Standard (His tag)

Recombinant Human KMT4/Dot1L Protein Standard (His tag)

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Recombinant Human KMT4/Dot1L Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.

別名を表示する

KIAA1814, KMT4, DOT1L, DOT1-like protein, Disruptor of telomeric silencing 1-like histone lysine methyltransferase, Histone H3-K79 methyltransferase, Lysine N-methyltransferase 4, H3-K79-HMTase

2 Images
Sandwich ELISA - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human KMT4/Dot1L Protein Standard (His tag) (AB323152)

SDS-PAGE analysis of ab323152 under reducing conditions for 2ug protein.

Key facts

精製度

>80% SDS-PAGE

発現系

Escherichia coli

タグ

His tag C-Terminus

アプリケーション

sELISA, SDS-PAGE

applications

生物活性

No

アクセッション番号

Q8TEK3

アニマルフリー

Yes

キャリアフリー

No

種

Human

バッファー組成

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
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配列情報

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"49.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8TEK3","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Histone methyltransferase that methylates 'Lys-79' of histone H3 (PubMed : 12123582, PubMed : 38503750, PubMed : 38129415). Nucleosomes are preferred as substrate compared to free histones (PubMed : 12123582, PubMed : 38503750). Binds to DNA (PubMed : 12628190, PubMed : 38503750).

配列の類似性

Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Histone methyltransferase that methylates 'Lys-79' of histone H3 (PubMed : 12123582, PubMed : 38503750, PubMed : 38129415). Nucleosomes are preferred as substrate compared to free histones (PubMed : 12123582, PubMed : 38503750). Binds to DNA (PubMed : 12628190, PubMed : 38503750).
See full target information DOT1L
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Abcam product promise

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