AB196419

Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus)

Name: Recombinant human KMT3B / NSD1 protein (ab196419) | Abcam
Brand: Abcam Limited
SKU: AB196419
Price: 243000 JPY
Availability: InStock

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Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 1849 to 2094 aa range, expressed in Escherichia coli, with >76%, suitable for SDS-PAGE, FuncS.

別名を表示する

ARA267, KMT3B, NSD1, Androgen receptor coactivator 267 kDa protein, Androgen receptor-associated protein of 267 kDa, H3-K36-HMTase, Lysine N-methyltransferase 3B, Nuclear receptor-binding SET domain-containing protein 1, NR-binding SET domain-containing protein

2 Images

Functional Studies - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)

FuncS

Supplier Data

Functional Studies - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)

Activity assay of ab196419. Assay was performed using a NSD1 Chemiluminescent Assay Kit with 400 μM S-adenosyl- methionine on biotinylated H3 peptide coated plates overnight at room temperature.

SDS-PAGE - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus) (AB196419)

SDS-PAGE of 3μg ab196419 (lane 1). Protein marker (lane 2).
4-20% gradient gel, coomassie staining.

Key facts

精製度

>76% SDS-PAGE

発現系

Escherichia coli

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Active

アクセッション番号

Q96L73

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.12% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"KAQKELRQLQEDRKNDKKPPPYKHIKVNRPIGRVQIFTADLSEIPRCNCKATDENPCGIDSECINRMLLYECHPTVCPAGGRCQNQCFSKRQYPEVEIFRTLQRGWGLRTKTDIKKGEFVNEYVGELIDEEECRARIRYAQEHDITNFYMLTLDKDRIIDAGPKGNYARFMNHCCQPNCETQKWSVNGDTRVGLFALSDIKAGTELTFNYNLECLGNGKTVCKCGAPNCSGFLGVRPKNQPIATEE","proteinLength":"Fragment","predictedMolecularWeight":"54 kDa","actualMolecularWeight":null,"aminoAcidEnd":2094,"aminoAcidStart":1849,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96L73","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

KMT3B also known as NSD1 is a histone methyltransferase enzyme with a significant role in chromatin modification. This protein adds methyl groups to histone H3 at lysine 36 (H3K36). With a molecular mass around 280 kDa NSD1 is mostly expressed in tissues such as the brain and skeletal muscle. It plays an integral role in controlling gene expression and maintaining genomic stability through its actions on chromatin.

Biological function summary

NSD1 modulates transcription regulation by interacting with other nuclear proteins. As a part of the epigenetic regulation mechanism it is involved in complex processes of genome organization. By methylating H3K36 NSD1 affects chromatin dynamics and influences transcriptional activation and repression. NSD1 supports the orchestration of transcriptional changes in response to cellular signals and environmental stimuli.

Pathways

NSD1 interfaces with transcriptional regulation and chromatin remodeling pathways. Its enzymatic action on H3K36 links NSD1 to pathways governing cell growth and differentiation. Through its interactions NSD1 associates with proteins like MLL and SETD2 highlighting its role in the regulation of gene expression and cellular developmental pathways.

NSD1's dysfunction associates with Sotos syndrome and acute myeloid leukemia. Mutations in NSD1 lead to overgrowth and developmental delay characteristics observed in Sotos syndrome. In leukemia disrupted methylation patterns arise from NSD1 alterations affecting proteins like NUP98 contributing to oncogenic transformation and malignancy progression.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Histone methyltransferase that dimethylates Lys-36 of histone H3 (H3K36me2). Transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context.

配列の類似性

Belongs to the class V-like SAM-binding methyltransferase superfamily.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information NSD1

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Recombinant human KMT3B / NSD1 protein (GST tag N-Terminus)