Recombinant Human KMT1E / SETDB1 protein (ab153023)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: ELISA, WB
製品の詳細
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製品名
Recombinant Human KMT1E / SETDB1 protein
KMT1E / SETDB1 タンパク質・ペプチド 製品一覧 -
発現系
Wheat germ -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SSLPGCIGLDAATATVESEEIAELQQAVVEELGISMEELRHFIDEELEKM DCVQQRKKQLAELETWVIQKESEVAHVDQLFDDASRAVTNCESLVKDFY -
領域
2 to 100 -
タグ
GST tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab153023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
Western blot
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
関連情報
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別名
- AU022152
- EC 2.1.1.43
- ERG-associated protein with SET domain
see all -
機能
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. -
組織特異性
Widely expressed. High expression in testis. -
配列類似性
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 MBD (methyl-CpG-binding) domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Contains 2 Tudor domains. -
ドメイン
The pre-SET, SET and post-SET domains are all required for methyltransferase activity. The 347-amino-acid insertion in the SET domain has no effect on the catalytic activity.
Isoform 2 lacks all domains required for histone methyltransferase activity. -
細胞内局在
Nucleus. Chromosome. Associated with non-pericentromeric regions of chromatin. Excluded from nucleoli and islands of condensed chromatin. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human KMT1E / SETDB1 protein (ab153023)ab153023 on a 12.5% SDS-PAGE stained with Coomassie Blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab153023 は論文での使用が確認できていません。
