Recombinant human KMT1B / SUV39H2 protein (ab80288)
Key features and details
- Expression system: Escherichia coli
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human KMT1B / SUV39H2 protein
KMT1B / SUV39H2 タンパク質・ペプチド 製品一覧 -
生理活性
Specific Activity: 38 pmol/min/mg.
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精製度
> 70 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
領域
26 to 350
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特性
Our Abpromise guarantee covers the use of ab80288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- FLJ23414
- H3 K9 HMTase 2
- H3-K9-HMTase 2
see all -
機能
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis. -
配列類似性
Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 1 chromo domain.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
ドメイン
Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin. -
細胞内局在
Nucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin. - Information by UniProt
画像
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Functional Studies - Recombinant human KMT1B / SUV39H2 protein (ab80288)Image showing specific activity of ab80288.
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SDS-PAGE - Recombinant human KMT1B / SUV39H2 protein (ab80288)10% SDS-PAGE showing ab80288 at approximately 63kDa (3µg).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab80288 は論文での使用が確認できていません。