Recombinant human Klotho protein (Active) (ab84072)
Key features and details
- Expression system: CHO cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
製品の詳細
-
製品名
Recombinant human Klotho protein (Active)
Klotho タンパク質・ペプチド 製品一覧 -
生理活性
Determined by the dose-dependent stimulation of the proliferation of murine NIH/3T3 cells.
ab84072 is effective in a concentration range of 0.5-2.0 μg/ml.
-
精製度
> 98 % SDS-PAGE.
> 98% HPLC analyses. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
CHO cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH -
予測される分子量
59 kDa -
領域
34 to 549
-
特性
Our Abpromise guarantee covers the use of ab84072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
HPLC
-
製品の状態
Lyophilized -
備考
Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Centrifuge vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage. Extended Storage: Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA). Store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
関連情報
-
別名
- Kl
- KLOT_HUMAN
- Klotho peptide
-
機能
May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. -
組織特異性
Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney. -
関連疾患
Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement. -
配列類似性
Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. -
ドメイン
Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab84072 は 8 報の論文で使用されています。
- Liu XY et al. STAT4-Mediated Klotho Up-Regulation Contributes to the Brain Ischemic Tolerance by Cerebral Ischemic Preconditioning via Inhibiting Neuronal Pyroptosis. Mol Neurobiol 61:2336-2356 (2024). PubMed: 37875707
- Tsai KD et al. Recombinant Klotho attenuates IFNγ receptor signaling and SAMHD1 expression through blocking NF-κB translocation in glomerular mesangial cells. Int J Med Sci 20:810-817 (2023). PubMed: 37213666
- Romero A et al. Pharmacological Blockade of NLRP3 Inflammasome/IL-1ß-Positive Loop Mitigates Endothelial Cell Senescence and Dysfunction. Aging Dis 13:284-297 (2022). PubMed: 35111374
- Jadhav S et al. A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma. PLoS One 16:e0245614 (2021). PubMed: 33481901
- Typiak M et al. Role of Klotho in Hyperglycemia: Its Levels and Effects on Fibroblast Growth Factor Receptors, Glycolysis, and Glomerular Filtration. Int J Mol Sci 22:N/A (2021). PubMed: 34360633
- Liu W et al. Recombinant Klotho protein enhances cholesterol efflux of THP-1 macrophage-derived foam cells via suppressing Wnt/ß-catenin signaling pathway. BMC Cardiovasc Disord 20:120 (2020). PubMed: 32138681
- Romero A et al. The angiotensin-(1-7)/Mas receptor axis protects from endothelial cell senescence via klotho and Nrf2 activation. Aging Cell 18:e12913 (2019). PubMed: 30773786
- Mazzotta C et al. Proangiogenic effects of soluble a-Klotho on systemic sclerosis dermal microvascular endothelial cells. Arthritis Res Ther 19:27 (2017). PubMed: 28183357