AB196415

Recombinant human KDM4E protein (GST tag N-Terminus)

Name: Recombinant human KDM4E protein (ab196415) | Abcam
Brand: Abcam Limited
SKU: AB196415
Price: 325000 JPY
Availability: InStock

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Recombinant human KDM4E protein (GST tag N-Terminus) is a Human Fragment protein, in the 2 to 337 aa range, expressed in Baculovirus infected Sf9 cells, with >37%, suitable for SDS-PAGE, FuncS.

別名を表示する

KDM4DL, KDM5E, KDM4E, Lysine-specific demethylase 4E, KDM4D-like protein, Lysine-specific demethylase 4D-like, [histone H3]-trimethyl-L-lysine(9) demethylase 4E

2 Images

Functional Studies - Recombinant human KDM4E protein (GST tag N-Terminus) (AB196415)

FuncS

Supplier Data

Functional Studies - Recombinant human KDM4E protein (GST tag N-Terminus) (AB196415)

Activity assay of ab196415.

SDS-PAGE - Recombinant human KDM4E protein (GST tag N-Terminus) (AB196415)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human KDM4E protein (GST tag N-Terminus) (AB196415)

10% SDS-PAGE analysis of ab196415 with Coomassie staining.

Key facts

精製度

>37% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Specific activity: 0.14 pmole/min/μg.

10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 μM α-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and ab196415 (100 – 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by detection.

アクセッション番号

B2RXH2

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.49% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"KSVHSSPQNTSHTIMTFYPTMEEFADFNTYVAYMESQGAHQAGLAKVIPPKEWKARQMYDDIEDILIATPLQQVTSGQGGVFTQYHKKKKAMRVGQYRRLANSKKYQTPPHQNFADLEQRYWKSHPGNPPIYGADISGSLFEESTKQWNLGHLGTILDLLEQECGVVIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKTWYVVPPEHGQHLERLARELFPDISRGCEAFLRHKVALISPTVLKENGIPFNCMTQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWIDYGKMASQCSCGESTVTFSMDPFVRIVQPESYELWKHRQ","proteinLength":"Fragment","predictedMolecularWeight":"65 kDa","actualMolecularWeight":null,"aminoAcidEnd":337,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"B2RXH2","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

保管に関する情報

Avoid freeze / thaw cycle

True

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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Histone demethylase that demethylates trimethylated 'Lys-9' of histone H3 into monomethylated form, thereby playing a central role in histone code (PubMed : 21914792, PubMed : 23451023). Also demethylates trimethylated 'Lys-56' of histone H3 (H3K9me3) to generate the monomethylated state (H3K56me1) (PubMed : 23451023).

配列の類似性

Belongs to the JHDM3 histone demethylase family.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information KDM4E

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Abcam product promise

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Recombinant human KDM4E protein (GST tag N-Terminus)