AB108575

Recombinant human Jagged1 protein (Fc Chimera Active)

Bioactive
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(2 Publications)

Recombinant human Jagged1 protein (Fc Chimera Active) is a Human Full Length protein, in the 1 to 1067 aa range, expressed in HEK 293 cells, with >90%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

別名を表示する

CD339, JAGL1, JAG1, Protein jagged-1, Jagged1, hJ1

2 Images

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Western blot - Recombinant human Jagged1 protein (Fc Chimera Active) (AB108575)

Induction of Hes-1 with the treatment of recombinant Human Jagged1-Fc (ab108575).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of ab108575 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

M : Marker
Lane 1 : ab108575, 0 min
Lane 2 : ab108575, 10 min
Lane 3 : ab108575, 30 min
Lane 4 : ab108575, 1 hr
Lane 5 : ab108575, 2 hr
Lane 6 : ab108575, 4 hr
Lane 7 : ab108575, 8 hr
Lane 8 : ab108575, 24 hr

All lanes:

Western blot - Recombinant human Jagged1 protein (Fc Chimera Active) (ab108575)

false

Functional Studies - Recombinant human Jagged1 protein (Fc Chimera Active) (AB108575)

FuncS

Supplier Data

Functional Studies - Recombinant human Jagged1 protein (Fc Chimera Active) (AB108575)

NIH/3T3 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin.

When the cells reached confluence, adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1μM, and 10μg/ml, respectively and continued for 2 days (day 0).

The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml of human Jagged1-Fc (ab108575) and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7

Key facts

精製度

>90% SDS-PAGE

エンドトキシンレベル

< 0.1 EU/µg

発現系

HEK 293 cells

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

Induction of HES in NIH/3T3 cells.

Inhibition of adipogenesis in NIH/3T3 cells.

アクセッション番号

P78504

アニマルフリー

キャリアフリー

Yes

種

Human

バッファー組成

Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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下記製品にもご興味をお持ちいただけるかもしれません:

AB267651

Human Jagged1 ELISA Kit

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製品の詳細

Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.

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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"150 kDa","actualMolecularWeight":null,"aminoAcidEnd":1067,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P78504","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.

Biological function summary

The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.

Pathways

Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.

Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.

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製品の性状

製品の状態

Liquid

補足情報

ab108575 is 0.2µm filtered

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一般的な情報

機能

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed : 18660822, PubMed : 20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed : 9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information JAG1

追加のターゲット

IGHG1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy oncolytics 23:51-64 PubMed34632050

2021

Knockdown of LRRN1 inhibits malignant phenotypes through the regulation of HIF-1α/Notch pathway in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yalu Zhang,Qiaofei Liu,Sen Yang,Quan Liao

Scientific reports 7:43926 PubMed28266574

2017

Notch1 Signaling Contributes to Hypoxia-induced High Expression of Integrin β1 in Keratinocyte Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Di Tang,Tiantian Yan,Junhui Zhang,Xupin Jiang,Dongxia Zhang,Yuesheng Huang

View all publications

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保証に関する詳細については利用規約をご確認ください。

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Recombinant human Jagged1 protein (Fc Chimera Active)