AB119452

Recombinant Human IRSp53 protein (His tag C-Terminus)

Name: Recombinant Human IRSp53 protein (His tag) (ab119452) | Abcam
Brand: Abcam Limited
SKU: AB119452
Price: 88000 JPY
Availability: InStock

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Recombinant Human IRSp53 protein (His tag C-Terminus) is a Human Full Length protein, in the 1 to 522 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

別名を表示する

BAR/IMD domain-containing adapter protein 2, Brain-specific angiogenesis inhibitor 1-associated protein 2, Fas ligand-associated factor 3, Insulin receptor substrate p53/p58, Insulin receptor substrate protein of 53 kDa, BAI-associated protein 2, BAI1-associated protein 2, Protein BAP2, FLAF3, IRS-58, IRSp53/58, IRSp53, Insulin receptor substrate p53, BAIAP2

1 Images

SDS-PAGE - Recombinant Human IRSp53 protein (AB119452)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human IRSp53 protein (AB119452)

15% SDS-PAGE showing ab119452 at approximately 58.4kDa (3μg).

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

Q9UQB8

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Protein previously known as BAIAP2.

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配列情報

[{"sequence":"MSLSRSEEMHRLTENVYKTIMEQFNPSLRNFIAMGKNYEKALAGVTYAAKGYFDALVKMGELASESQGSKELGDVLFQMAEVHRQIQNQLEEMLKSFHNELLTQLEQKVELDSRYLSAALKKYQTEQRSKGDALDKCQAELKKLRKKSQGSKNPQKYSDKELQYIDAISNKQGELENYVSDGYKTALTEERRRFCFLVEKQCAVAKNSAAYHSKGKELLAQKLPLWQQACADPSKIPERAVQLMQQVASNGATLPSALSASKSNLVISDPIPGAKPLPVPPELAPFVGRMSAQESTPIMNGVTGPDGEDYSPWADRKAAQPKSLSPPQSQSKLSDSYSNTLPVRKSVTPKNSYATTAENKTLPRSSSMAAGLERNGRMRVKAIFSHAAGDNSTLLSFKEGDLITLLVPEARDGWHYGESEKTKMRGWFPFSYTRVLDSDGSDRLHMSLQQGKSSSTGNLLDKDDLAIPPPDYGAASRAFPAQTASGFKQRPYSVAVPAFSQGLDDYGARSMSSGSGTLVSTVVEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"58.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":522,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9UQB8","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

IRSp53 also known as BAIAP2 (Brain-Specific Angiogenesis Inhibitor 1-Associated Protein 2) is a protein with a molecular mass of approximately 58 kDa. It acts as an important regulator of actin cytoskeleton dynamics facilitating the formation of membrane protrusions like filopodia and lamellipodia. IRSp53 is expressed ubiquitously in various tissues with higher levels observed in the brain. It contains several domains including the IMD (I-BAR) domain which is involved in membrane curvature and actin binding.

Biological function summary

IRSp53 participates in the modulation of cell shape and motility. It is a component of the protein complexes that couple membrane dynamics to cytoskeleton remodeling. Through its interaction with proteins like CDC42 and Rac1 IRSp53 guides the rearrangement of actin filaments affecting cell movement and shape. These abilities make it significant for cellular processes requiring changes in cell structure such as neurodevelopment and wound healing.

Pathways

IRSp53 is central to the regulation of cell motility pathways and signaling processes. It plays an important role in the WAVE (WASP family verprolin-homologous protein) regulatory complex which influences actin polymerization. The protein interacts with Rac1 and N-WASP to mediate pathways leading to the formation of actin-rich structures. This modulation of actin dynamics links IRSp53 to the broader MAPK/ERK signaling pathway impacting cellular proliferation and differentiation.

IRSp53 has been linked to neurological and oncological conditions. Alterations in its expression or function have connections with autism spectrum disorders and glioblastoma. In these disorders IRSp53's interaction with proteins like BAIAP2L1 can influence signal transduction and cytoskeletal organization which may contribute to disease pathology. Understanding IRSp53's roles could offer insights into its potential as a therapeutic target.

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製品の性状

製品の状態

Liquid

補足情報

ab119452 was purified using conventional chromatography techniques.

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一般的な情報

機能

Adapter protein that links membrane-bound small G-proteins to cytoplasmic effector proteins. Necessary for CDC42-mediated reorganization of the actin cytoskeleton and for RAC1-mediated membrane ruffling. Involved in the regulation of the actin cytoskeleton by WASF family members and the Arp2/3 complex. Plays a role in neurite growth. Acts syngeristically with ENAH to promote filipodia formation. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection. Participates in actin bundling when associated with EPS8, promoting filopodial protrusions.

翻訳後修飾

Phosphorylated on tyrosine residues by INSR in response to insulin treatment.

細胞内局在性

Cytoskeleton

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Brain-specific angiogenesis inhibitor 1-associated protein 2

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Abcam product promise

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Recombinant Human IRSp53 protein (His tag C-Terminus)