AB318948

Recombinant Human IRF5 Protein (His tag)

Name: Recombinant Human IRF5 Protein (His tag) (ab318948) | Abcam
Brand: Abcam Limited
SKU: AB318948
Price: 48000 JPY
Availability: InStock

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Recombinant Human IRF5 Protein (His tag) is a Human Full Length protein, expressed in Escherichia coli, with >95%, 0.005 EU/µg endotoxin level, suitable for Mass Spec, SDS-PAGE.

別名を表示する

Interferon regulatory factor 5, IRF-5, IRF5

2 Images

Mass Spectrometry - Recombinant Human IRF5 Protein (His tag) (AB318948)

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human IRF5 Protein (His tag) (AB318948)

Mass determination by ESI-TOF. Predicted MW is 57110 Da (+/- 10 Da by ESI-TOF). Observed MW is 57108.97 Da.

SDS-PAGE - Recombinant Human IRF5 Protein (His tag) (AB318948)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human IRF5 Protein (His tag) (AB318948)

SDS-PAGE analysis of ab318948 under reducing conditions for 2ug protein.

Key facts

精製度

>95% HPLC

エンドトキシンレベル

0.005 EU/µg

発現系

Escherichia coli

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

質量分析

LC-MS/MS

アクセッション番号

Q13568

アニマルフリー

Yes

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.7293% Sodium chloride, 0.3002% Tris HCl, 0.0148% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ","proteinLength":"Full Length","predictedMolecularWeight":"57.11 kDa","actualMolecularWeight":"57.12 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q13568","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

IRF5 or Interferon Regulatory Factor 5 is a member of the IRF family of transcription factors. This protein weighs approximately 56 kDa and is expressed in cells of the immune system including B cells dendritic cells and macrophages. IRF5 plays an important role in regulating the transcription of interferon-stimulated genes following a viral infection. It acts by binding to specific DNA sequences initiating transcription processes that are important for the antiviral response.

Biological function summary

IRF5 influences the immune response through the regulation of cytokine production. It activates genes involved in producing pro-inflammatory cytokines such as TNF-α and IL-6 which are important for clearing viral infections and activating immune cells. IRF5 does not usually form part of a protein complex but it interacts closely with TRAF6 and MyD88 for its activation. Its pivotal role in cytokine production highlights its importance in controlling inflammation.

Pathways

IRF5 is an important component of the Toll-like receptor (TLR) signaling pathway and plays a role in the MyD88-dependent pathway. In this context IRF5 gets activated by TLRs interacting with MyD88 and TAK1 to enhance the transcription of important genes for immune response. It is also closely connected to the IRF3 protein which shares similar activation pathways and can similarly initiate an antiviral state.

Defects or dysregulation in IRF5 function are linked to autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE IRF5 influences autoantibody production contributing to disease development. It works alongside other regulatory proteins involved in autoimmunity including IFN-α and STAT4 which also play significant roles in modulating immune responses. Understanding IRF5's role in these conditions could present new opportunities for therapeutic interventions using IRF5 inhibitors.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PubMed : 11303025, PubMed : 15695821, PubMed : 22412986, PubMed : 25326418, PubMed : 32433612). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (By similarity). Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway (By similarity). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed : 33440148).

配列の類似性

Belongs to the IRF family.

翻訳後修飾

Phosphorylation of serine and threonine residues by IKBKB in a C-terminal autoinhibitory region, stimulates dimerization, transport into the nucleus, assembly with the coactivator CBP/EP300 and initiation of transcription.. 'Lys-63'-linked polyubiquitination by TRAF6 is required for activation.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information IRF5

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Abcam product promise

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Recombinant Human IRF5 Protein (His tag)