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AB63177

Recombinant ヒト IPL-1/STK13/Aurora C protein (GST tag N-Terminus)

Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus)

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Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

別名を表示する

AIE2, AIK3, AIRK3, ARK3, STK13, AURKC, Aurora kinase C, Aurora 3, Aurora/IPL1-related kinase 3, Aurora/IPL1/Eg2 protein 2, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase aurora-C, ARK-3, Aurora-related kinase 3

4 Images
Functional Studies - Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) (AB63177)
  • FuncS

Unknown

Functional Studies - Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) (AB63177)

The specific activity of IPL-1/STK13/Aurora C (ab63177) was determined to be 65 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) (AB63177)
  • FuncS

Unknown

Functional Studies - Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) (AB63177)

Kinase activity plot

SDS-PAGE - Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) (AB63177)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) (AB63177)

SDS PAGE analysis of ab63177

SDS-PAGE - Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) (AB63177)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human IPL-1/STK13/Aurora C protein (GST tag N-Terminus) (AB63177)

ab63177 on SDS-PAGE, MW ~61 kDa

Key facts

精製度

>80% Densitometry

Affinity purified.

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

Specific activity 65nmol/min/mg

アクセッション番号

Q9UQB9

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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組合せ製品

Primary Antibodies

AB46783

Anti-IPL-1/STK13/Aurora C antibody [EP1011Y]

RabMAb|Recombinant

Western blot - Anti-IPL-1/STK13/Aurora C antibody [EP1011Y] (AB46783)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase assay.</p>" } } }
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製品の詳細

ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
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配列情報

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UQB9","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

IPL-1 also known as STK13 or Aurora C is a serine/threonine protein kinase with a molecular mass of approximately 39 kDa. It expresses mainly in testis and ovarian tissue playing a significant role in cell division. As a member of the Aurora kinase family IPL-1 functions mechanistically in regulating the centrosome during cell cycle progression ensuring the proper segregation of chromosomes during mitosis and meiosis. The protein undergoes phosphorylation to activate its kinase activity which is essential for its role in chromatin modification and assembly of microtubules.
Biological function summary

IPL-1 functions in chromosomal alignment and cytokinesis during cell division. It forms part of the chromosomal passenger complex (CPC) along with INCENP Survivin and Borealin. This complex is essential for correcting errors in chromosome segregation. IPL-1's activity helps maintain genomic stability by ensuring that cells divide correctly. Given its role in the chromosomal passenger complex IPL-1 is critical in monitoring and controlling the accurate distribution of genetic material between daughter cells.

Pathways

IPL-1 is intricately involved in cellular processes like the mitotic spindle checkpoint and the regulation of the mitotic cell cycle. It interacts with other proteins such as Aurora B which shares similar functions in the mitotic pathway. IPL-1 helps coordinate the activities of these pathways and proteins to ensure orderly cell division. Through these pathways IPL-1 influences the balance of cell proliferation and cell death which is important for normal organismal development and function.

Misregulation of IPL-1 is associated with cancers including ovarian and testicular cancer. Aberrant expression or activation of IPL-1 can lead to chromosomal instability contributing to tumorigenesis. Additionally the overexpression of Aurora kinase family members including IPL-1 often co-occurs with Cyclin B1 dysregulation in cancer cells. The connection between IPL-1 and Cyclin B1 highlights potential therapeutic targets for cancer treatment as researchers continue to explore strategies for inhibiting IPL-1 to restore normal cell cycle control in tumor cells.
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一般的な情報

機能

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Also plays a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.

配列の類似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

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製品プロトコール

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ターゲットの情報

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Also plays a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.
See full target information AURKC
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