Recombinant human Interferon alpha 1 protein (Active) (ab48750)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Sandwich ELISA
製品の詳細
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製品名
Recombinant human Interferon alpha 1 protein (Active)
Interferon alpha 1 タンパク質・ペプチド 製品一覧 -
生理活性
Interferon alpha 1 (ab48750) has the activity of 4 X 105 unit/mg (or 4 X 105 unit/ml).
This activity has been tested by evaluating anti-proliferation with Daudi cells. For Further information see Kim et al., 2009.
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精製度
> 95 % SDS-PAGE.
This protein was purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
CDLPETHSLD NRRTLMLLAQ MSRISPSSCL MDRHDFGFPQ EEFDGNQFQK APAISVLHEL IQQIFNLFTT KDSSAAWDED LLDKFCTELY QQLNDLEACV MQEERVGETP LMNADSILAV KKYFRRITLY LTEKKYSPCA WEVVRAEIMR SLSLSTNLQE RLRRKE -
予測される分子量
20 kDa -
領域
24 to 189 -
配列の追加情報
Full length mature protein, without the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab48750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Sandwich ELISA
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製品の状態
Liquid -
備考
Concentration determined by Bradford assay.
Endotoxin levels determined by LAL method.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- IFL
- IFN
- IFN alpha
see all -
機能
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
配列類似性
Belongs to the alpha/beta interferon family. -
細胞内局在
Secreted. - Information by UniProt
画像
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Sandwich ELISA - Recombinant human Interferon alpha 1 protein (ab48750)
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SDS-PAGE - Recombinant human Interferon alpha 1 protein (ab48750)3ug of reduced ab48750 on SDS-PAGE, stained with Coomassie Blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab48750 は 9 報の論文で使用されています。
- Khatri A et al. JAK-STAT activation contributes to cytotoxic T cell-mediated basal cell death in human chronic lung allograft dysfunction. JCI Insight 8:N/A (2023). PubMed: 36946463
- Constantin D et al. APOBEC3C, a nucleolar protein induced by genotoxins, is excluded from DNA damage sites. FEBS J 289:808-831 (2022). PubMed: 34528388
- Bencze D et al. Interactions between the NLRP3-Dependent IL-1β and the Type I Interferon Pathways in Human Plasmacytoid Dendritic Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36293012
- Guo C et al. Single-cell transcriptome profiling and chromatin accessibility reveal an exhausted regulatory CD4+ T cell subset in systemic lupus erythematosus. Cell Rep 41:111606 (2022). PubMed: 36351407
- Cotter KA et al. Mapping of m6A and Its Regulatory Targets in Prostate Cancer Reveals a METTL3-Low Induction of Therapy Resistance. Mol Cancer Res 19:1398-1411 (2021). PubMed: 34088870
- Lea AJ et al. Genome-wide quantification of the effects of DNA methylation on human gene regulation. Elife 7:N/A (2018). PubMed: 30575519
- Alagu J et al. Tumor Suppressor p14ARF Enhances IFN-?-Activated Immune Response by Inhibiting PIAS1 via SUMOylation. J Immunol 201:451-464 (2018). PubMed: 29848755
- Moriyama M et al. Two Conserved Amino Acids within the NSs of Severe Fever with Thrombocytopenia Syndrome Phlebovirus Are Essential for Anti-interferon Activity. J Virol 92:N/A (2018). PubMed: 30021900
- Sun B et al. Dengue virus activates cGAS through the release of mitochondrial DNA. Sci Rep 7:3594 (2017). PubMed: 28620207