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Recombinant human IL2 Receptor beta/p75 protein (Fc Chimera) (ab84068)

Submit a review Q&A (3)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE

製品の詳細

  • 製品名

    Recombinant human IL2 Receptor beta/p75 protein (Fc Chimera)
    IL2 Receptor beta/p75 タンパク質・ペプチド 製品一覧

  • 生理活性

    ab84068 bound to protein A sepharose beads is able to pull down its ligand, IL2.

  • 精製度

    > 95 % SDS-PAGE.

  • 発現系

    HEK 293 cells

  • アクセッション番号

    P14784

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      Theoretical Sequence: AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWP DRRRWNQ TCELLPVSQASWACNLILGAPDSQKLTTVDIVTLRVLCREG VRWRVMAI QDFKPFENLRLMAPISLQVVHVETHRCNISWEISQASHYF ERHLEFEART LSPGHTWEEAPLLTLKQKQEWICLETLTPDTQYEFQVR VKPLQGEFTTW SPWSQPLAFRTKPAALGIPKVDKKVEPKSCDKTHTCP PCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKF NWYVDGVEVHNAK TKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKV SNKALPAPIEKTIS KAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKG FYPSDIAVEWESNG QPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQ GNVFSCSVMHEALH NHYTQKSLSLSPGK

    • 領域

      27 to 237

    • 配列の追加情報

      DNA sequence encoding the signal peptide and extracellular domain of human IL 2R beta (aa 1-237) was fused to the Fc region of human IgG1 (aa93-330). Protein expressed in modified human 293 cells.

特性

Our Abpromise guarantee covers the use of ab84068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

  • 製品の状態

    Lyophilized

  • 備考

    ab84068 bound to protein A sepharose beads is able to pull down its ligand, IL2.

     This product was previously labelled as IL2 Receptor beta

     

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Constituents: 1% Human serum albumin, 10% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

関連情報

  • 別名

    • CD122
    • CD122 antigen
    • High affinity IL 2 receptor beta subunit
    • High affinity IL 2 receptor subunit beta
    • High affinity IL-2 receptor subunit beta
    • IL-2 receptor subunit beta
    • IL-2R subunit beta
    • IL-2RB
    • IL15RB
    • IL2 receptor
    • IL2RB
    • IL2RB_HUMAN
    • Interleukin 2 receptor beta
    • Interleukin-2 receptor subunit beta
    • P70 75
    • P70-75
    • P7075
    • p75
    see all

  • 機能

    Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

  • 配列類似性

    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.

  • ドメイン

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

  • 細胞内局在

    Membrane.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab84068 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab84068 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

Does the antibody bind to the extracellular domain of CD122 and is it
known whether that antibody leave the receptor fully functional (IL-2
binding, internalising) once bound?
All the best

Read More
Answer

The ab25227 antibody specificity on the datasheet shows the products does not block the receptor so it means the receptor is fully functional.The epitope of this antibody has not been determined so I am sorry we are unable to confirm which part of domain this antibody binds.

The antibody ab171228; https://www.abcam.com/il2-receptor-beta-tm-beta1-antibody-low-endotoxin-azide-free-ab171228.html is known to inhibit the iL2 to receptor binding.

Read More

Question

Inquiry: Hi, I'm looking to see if it is at all possible to get this material (ab84068) without added albumin. The carrier interferes with the assay I am trying to do. I don't need much, and I am local, and I am willing to have the protein in whatever format is easiest, as long as there is no added protein. Can you give me an idea if this is at all possible? Thanks, Heather

Read More
Answer

Thank you for contacting us.

We cuurrently do not have this product in an albumin free state. The albumin is added by the originating lab as stabilizer and lyophilised prior to shipping. I am sorry for any inconvenience this may cause.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Does this protein retain function, in particular can the IL2 receptor beta still bind protein?

Read More
Answer

This purifed IL-2beta receptor protein does bind IL-2 as shown by its ability to precipitate it whenab84068 is bound to protein A beads in an immunoprecipitation experiment. However it is not a fully functional receptor as ab84068 only contains the extracellular region of the protein.



I hope this helps. If you have any further questions please feel free to contact me again.

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