Recombinant human IL2 Receptor beta/p75 protein (Active) (ab174003)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Biological Activity, ELISA, Functional Studies
製品の詳細
-
製品名
Recombinant human IL2 Receptor beta/p75 protein (Active)
IL2 Receptor beta/p75 タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in SPR assay. ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 0.525 µM.
Measured by its binding ability in SPR assay. ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-15, Tag Free with an affinity constant of 21.7 nM.
Measured by its binding ability in BLI assay. Loaded ab174003 on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e).
Measured by its inhibitory action in a cell based assay. ab174003 inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 µg/mL.
-
精製度
> 90 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWPDRRRWNQTCEL LPVSQASWACNLILGAPDSQKLTTVDIVTLRVLCREGVRWRVMAIQDFKP FENLRLMAPISLQVVHVETHRCNISWEISQASHYFERHLEFEARTLSPGH TWEEAPLLTLKQKQEWICLETLTPDTQYEFQVRVKPLQGEFTTWSPWSQP LAFRTKPAALGKD -
予測される分子量
25 kDa including tags -
領域
27 to 239 -
タグ
His tag C-Terminus -
配列の追加情報
NM_000878. Extracellular domain.
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab174003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Biological Activity
ELISA
Functional Studies
-
製品の状態
Lyophilized -
備考
This product was previously labelled as IL2 Receptor beta.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Reconstitute for long term storage.
pH: 7.40
Constituents: 5% Trehalose, 95% PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
関連情報
-
別名
- CD122
- CD122 antigen
- High affinity IL 2 receptor beta subunit
see all -
機能
Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. -
配列類似性
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
細胞内局在
Membrane. - Information by UniProt
画像
-
Biological activity - Recombinant human IL2 Receptor beta/p75 protein (ab174003)
ab174003 inhibits the IL-15-dependent proliferation of Mo7e cells. The EC50 for this effect is 0.38-0.45 µg/mL.
-
Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (ab174003)Loaded ab174003 on HIS1K Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 0.46 μM as determined in BLI assay (ForteBio Octet Red96e).
-
Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (ab174003)ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-15, Tag Free with an affinity constant of 21.7 nM as determined in a SPR assay (Biacore T200).
-
Functional Studies - Recombinant human IL2 Receptor beta/p75 protein (ab174003)ab174003 captured on CM5 chip via anti-His antibody, can bind Human IL-2, Tag Free with an affinity constant of 0.525 µM as determined in a SPR assay (Biacore T200).
-
SDS-PAGE - Recombinant human IL2 Receptor beta/p75 protein (ab174003)SDS-PAGE of reduced ab174003 stained overnight with Coomassie Blue. The protein migrates as 35-45 kDa due to glycosylation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab174003 は論文での使用が確認できていません。
