Recombinant Human IL-33 protein (Active) (ab281811)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE, MS, Cell Culture, Biological Activity
製品の詳細
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製品名
Recombinant Human IL-33 protein (Active)
IL-33 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent proliferation of D10S cells. ED50 is ≤ 1.70 ng/ml, corresponding to a specific activity of 5.9 x 105 units/mg.
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精製度
> 95 % SDS-PAGE.
Purity >=95% by HPLC. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
AFGISGVQKY TRALHDSSIT GISPITEYLA SLSTYNDQSI TFALEDESYE IYVEDLKKDE KKDKVLLSYY ESQHPSNESG DGVDGKMLMV TLSPTKDFWL HANNKEHSVE LHKCEKPLPD QAFFVLHNMH SNCVSFECKT DPGVFIGVKD NHLALIKVDS SENLCTENIL FKLSET -
予測される分子量
20 kDa -
実際の分子量
20 kDa -
分子量情報
Predicted MW is 19883.29 Da (+/- 10 Da by ESI-TOF). Observed MW is 19884.54 Da. -
領域
95 to 270 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide and pro-peptide.
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特性
Our Abpromise guarantee covers the use of ab281811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
HPLC
SDS-PAGE
Mass Spectrometry
Cell Culture
Biological Activity
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product
関連情報
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別名
- C9orf26
- CHROMOSOME 9 OPEN READING FRAME 26
- DKFZp586H0523
see all -
機能
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). -
組織特異性
Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. -
配列類似性
Belongs to the IL-1 family. Highly divergent. -
ドメイン
The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association. -
翻訳後修飾
The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. -
細胞内局在
Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). - Information by UniProt
画像
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Biological Activity - Recombinant Human IL-33 protein (ab281811)Recombinant Human IL-33 protein was determined to be fully biologically active by the dose dependent proliferation of D10S cells. ED50 is ≤ 1.70 ng/ml, corresponding to a specific activity of 5.9 x 10⁵ units/mg.
Cell based assay testing is performed on the first lot of the protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3425563-1.
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SDS-PAGE - Recombinant Human IL-33 protein (ab281811)SDS-PAGE analysis of ab281811.
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Mass Spectrometry - Recombinant Human IL-33 protein (ab281811)ESI-TOF analysis of ab281811.
Predicted MW is 19883.29 Da (+/- 10 Da by ESI-TOF). Observed MW is 19884.54 Da.
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HPLC - Recombinant Human IL-33 protein (ab281811)HPLC analysis of ab281811.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab281811 は論文での使用が確認できていません。
