Recombinant human IL-27-A+EBI3 protein (Active) (ab245785)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
製品の詳細
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製品名
Recombinant human IL-27-A+EBI3 protein (Active) -
生理活性
Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 range is 400-500 ng/ml.
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精製度
> 90 % SDS-PAGE.
>90% by HPLC. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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アミノ酸配列 1
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生物種
Human -
配列
FPRPPGRPQLSLQELRREFTVSLHLARKLLSEVRGQAHRFAESHLPGVNL YLLPLGEQLPDVSLTFQAWRRLSDPERLCFISTTLQPFHALLGGLGTQGR WTNMERMQLWAMRLDLRDLQRHLRFQVLAAGFNLPEEEEEEEEEEEEERK GLLPGALGSALQGPAQVSWPQLLSTYRLLHSLELVLSRAVRELLLLSKAG HSVWPLGFPTLSPQP -
予測される分子量
25 kDa -
領域
29 to 243 -
配列の追加情報
Full-length mature chain lacking the signal peptide.
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アミノ酸配列 2
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生物種
Human -
配列
RKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGM AARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVP FITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYK RQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLP ATATMSLGK -
領域
21 to 229 -
配列の追加情報
Full-length mature chain lacking the signal peptide
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特性
Our Abpromise guarantee covers the use of ab245785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
HPLC
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製品の状態
Lyophilized -
備考
ab245785 is a non-disulfide-linked, heterodimeric protein composed of a 209 amino acid length EBI3 subunit and a 215 amino acid length IL-27-p28 subunit, for a total sequence length of 424 amino acid residues. The calculated molecular weight of the associated IL-27 subunits is 47.8 kDa.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 6.5 + 100mM
NaCl.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Centrifuge vial prior to opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. Store at 2°C to 8°C for 1 week, or prepare for extended storage.
関連情報
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別名
- IL27-A+EBI3
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細胞内局在
IL27-A: Secreted. Does not seem to be secreted without coexpression of EBI3. EBI3: Secreted.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab245785 は論文での使用が確認できていません。
