Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
Bioactive grade

Recombinant human IL-23 protein (Active) (ab109153)

Submit a review Submit a question
Functional Studies - Recombinant human IL-23 protein (ab109153)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 0.060 Eu/µg
    • Active: Yes
    • Tags: DDDDK tag N-Terminus
    • Suitable for: Functional Studies, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant human IL-23 protein (Active)
      IL-23 タンパク質・ペプチド 製品一覧

    • 生理活性

      Induction of IL-17 secretion by mouse splenocytes.

    • 精製度

      > 90 % SDS-PAGE.

    • エンドトキシン・レベル

      < 0.060 Eu/µg

    • 発現系

      HEK 293 cells

    • アクセッション番号

      Q9NPF7

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCSGSTS GSGKPGSGEG STKGRAVPGG SSPAWTQCQQ LSQKLCTLAW SAHPLVGHMD LREEGDEETT NDVPHIQCGD GCDPQGLRDN SQFCLQRIHQ GLIFYEKLLG SDIFTGEPSL LPDSPVGQLH ASLLGLSQLL QPEGHHWETQ QIPSLSPSQP WQRLLLRFKI LRSLQAFVAV AARVFAHGAA TLSP

      • 予測される分子量

        70 kDa including tags

      • 領域

        23 to 328

      • タグ

        DDDDK tag N-Terminus

      • 配列の追加情報

        Fused at the C-terminus to a 18 aa linker peptide (GSTSGSGKPGSGEGSTKG) and to the IL23 p19 peptide (aa 20-189) and tagged at the N-terminus to a DDDDK-tag. IL-12p40 (human) Swissprot: P29460

    特性

    Our Abpromise guarantee covers the use of ab109153 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Functional Studies

      SDS-PAGE

    • 製品の状態

      Lyophilized

    • 備考

      Induction of IL17 secretion by Mouse splenocytes.

      PBS containing at least 0.1% BSA should be used for further dilutions.

      Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml.
      Add 1X PBS to the desired protein concentration.

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section.

      Constituent: 99% PBS

      0.2µm-filtered.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • 再構成
      Reconstitute 10µg vial with 100 µl sterile water to a concentration of 0.1mg/ml. Add 1X PBS to the desired protein concentration.

    関連情報

    • 別名

      • IL 23
      • IL 23 A
      • IL 23 subunit alpha
      • IL 23A
      • IL 23p19
      • IL-23 subunit alpha
      • IL-23-A
      • IL-23p19
      • IL12B
      • IL23
      • Il23a
      • IL23A_HUMAN
      • IL23P19
      • interleukin 12B
      • Interleukin 23 alpha subunit p19
      • Interleukin 23 p19 subunit
      • interleukin 23 subunit alpha
      • interleukin 23 subunit p19
      • interleukin six, G CSF related factor
      • Interleukin-23 subunit alpha
      • Interleukin-23 subunit p19
      • JKA3 induced upon T cell activation
      • MGC79388
      • P19
      • SGRF
      see all

    • 機能

      Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

    • 組織特異性

      Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).

    • 配列類似性

      Belongs to the IL-6 superfamily.

    • 発生段階

      Expressed by newborns dendritic cells.

    • 細胞内局在

      Secreted. Secreted upon association with IL12B.
    • Information by UniProt

    画像

    • Functional Studies - Recombinant human IL-23 protein (ab109153)
      Functional Studies - Recombinant human IL-23 protein (ab109153)

      IL17 secretion by Mouse splenocytes
      Mononuclear cells isolated from Mouse spleen were cultured with Mouse IL23 in presence or absence of various concentrations of ab109153 for 3 days. Levels of IL17 accumulated in culture supernatants were measured using ELISA.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab109153 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109153 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab109153.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com