AB280331

Recombinant human IL-22 protein (Active)

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Recombinant human IL-22 protein (Active) is a Human Full Length protein, in the 34 to 179 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

別名を表示する

ILTIF, ZCYTO18, UNQ3099/PRO10096, IL22, Interleukin-22, IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-TIF

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Functional Studies - Recombinant human IL-22 protein (Active) (AB280331)

FuncS

Supplier Data

Functional Studies - Recombinant human IL-22 protein (Active) (AB280331)

Functional analysis of ab280331.

Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.

ED₅₀ is 390 pg/ml.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3377996-1

Mass Spectrometry - Recombinant human IL-22 protein (Active) (AB280331)

Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human IL-22 protein (Active) (AB280331)

Mass determination by ESI-TOF.

Predicted MW is 16806.39 Da (+/- 10 Da by ESI-TOF). Observed MW is 16809.33

HPLC - Recombinant human IL-22 protein (Active) (AB280331)

HPLC

Supplier Data

HPLC - Recombinant human IL-22 protein (Active) (AB280331)

HPLC analysis of ab280331.

SDS-PAGE - Recombinant human IL-22 protein (Active) (AB280331)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human IL-22 protein (Active) (AB280331)

SDS-PAGE analysis of ab280331.

Key facts

精製度

>95% SDS-PAGE

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

アプリケーション

Mass Spec, HPLC, Cell Culture, FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Fully biologically active determined by the dose dependent induction of IL-10 secretion in COLO-205 cells.

ED₅₀ is 390 pg/ml.

アクセッション番号

Q9GZX6

アニマルフリー

Yes

キャリアフリー

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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配列情報

[{"sequence":"APISSHCRLDKSNFQQPYITNRTFMLAKEASLADNNTDVRLIGEKLFHGVSMSERCYLMKQVLNFTLEEVLFPQSDRFQPYMQEVVPFLARLSNRLSTCHIEGDDLHIQRNVQKLKDTVKKLGESGEIKAIGELDLLFMSLRNACI","proteinLength":"Full Length","predictedMolecularWeight":"16.81 kDa","actualMolecularWeight":"16.81 kDa","aminoAcidEnd":179,"aminoAcidStart":34,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9GZX6","tags":[]}]

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

Ambient

長期保存温度

Ambient

True

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製品の性状

製品の状態

Lyophilized

補足情報

=95% Purity by HPLC

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一般的な情報

機能

Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed : 17204547). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed : 17204547). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB (PubMed : 10875937, PubMed : 18599299). Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways (PubMed : 25793261, PubMed : 31311100). Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (By similarity). Promotes epithelial cell spreading (By similarity).

配列の類似性

Belongs to the IL-10 family.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information IL22

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Abcam product promise

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Recombinant human IL-22 protein (Active)