Recombinant human IL-16 protein (ab84082)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human IL-16 protein
IL-16 タンパク質・ペプチド 製品一覧 -
生理活性
Biological Activity: Determined by its ability ot chemoattract Human CD4+ T-Lymphocytes using a concentration range of 50.0-100.0 ng/ml. -
精製度
> 95 % SDS-PAGE.
Purity is greater than 98% by SDS-PAGE gel and HPLC analyses. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SAASASAASD VSVESTAEAT VCTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETVQPG DEILQLGGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QSKETTAAGD S
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特性
Our Abpromise guarantee covers the use of ab84082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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再構成Reconstituted ab84082 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.
関連情報
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別名
- HGNC:5980
- HsT19289
- IL-16
see all -
機能
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. -
組織特異性
Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation. -
配列類似性
Contains 4 PDZ (DHR) domains. -
翻訳後修飾
Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. -
細胞内局在
Cytoplasm; Cytoplasm. Nucleus and Secreted. - Information by UniProt
画像
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Functional Studies - Recombinant human IL-16 protein (ab84082)The migration of human peripheral lymphocytes in response to human IL-16.
Human peripheral blood lymphocytes were used in the chemotaxis assay at a final concentration of 5x106/ml.
*p<0.001 compared to binding medium.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab84082 は論文での使用が確認できていません。
