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Bioactive grade

Recombinant human IL-1 beta protein (Active) (ab9617)

Submit a review Q&A (5)References (6)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Endotoxin level: < 1.000 Eu/µg
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE, ELISA, HPLC

Achieve higher consistency and quality standards with a premium grade bioactive protein

Product image
Recombinant human IL-1 beta protein (Active) (ab259387)
  • High batch-to-batch consistency
  • Optimal bioactivity
  • Guaranteed identical to human native proteins
  • >95% purity
  • Ultra-low endotoxin levels: <0.005 Eu/µg
  • Carrier and tag free

製品の詳細

  • 製品名

    Recombinant human IL-1 beta protein (Active)
    IL-1 beta タンパク質・ペプチド 製品一覧

  • 生理活性

    The ED50,as determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells, is ≤ 1.0 pg/ml, corresponding to a specific activity of ≥ 1 x 109 units/mg.

  • 精製度

    > 98 % SDS-PAGE.
    > 98% HPLC. Sterile filtered.

  • エンドトキシン・レベル

    < 1.000 Eu/µg

  • 発現系

    Escherichia coli

  • アクセッション番号

    P01584

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

    • 予測される分子量

      17 kDa

    • 領域

      117 to 269

    • 配列の追加情報

      Full-length mature protein, minus the propeptide.

特性

Our Abpromise guarantee covers the use of ab9617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

    SDS-PAGE

    ELISA

    HPLC

  • 製品の状態

    Lyophilized

  • 備考

    Licensed under European Patent 165,654.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituents: 0.3% Sodium chloride, 0.16% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

関連情報

  • 別名

    • Catabolin
    • H1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • interleukin 1, beta
    • Interleukin-1 beta
    • OAF
    • Osteoclast activating factor
    • OTTHUMP00000162031
    • Preinterleukin 1 beta
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all

  • 機能

    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

  • 組織特異性

    Expressed in activated monocytes/macrophages (at protein level).

  • 配列類似性

    Belongs to the IL-1 family.

  • 翻訳後修飾

    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

  • 細胞内局在

    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (6)

ab9617 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9617 は 6 報の論文で使用されています。

  • Hsueh YH  et al. Therapeutic effect of induced pluripotent stem cell -derived extracellular vesicles in an in vitro and in vivo osteoarthritis model. J Orthop Translat 38:141-155 (2023). PubMed: 36381245
  • Sterling JK  et al. Inflammatory adipose activates a nutritional immunity pathway leading to retinal dysfunction. Cell Rep 39:110942 (2022). PubMed: 35705048
  • Sang W  et al. METTL3 involves the progression of osteoarthritis probably by affecting ECM degradation and regulating the inflammatory response. Life Sci 278:119528 (2021). PubMed: 33894271
  • Chen Z  et al. CRISPR/Cas9-based liver-derived reporter cells for screening of mPGES-1 inhibitors. J Enzyme Inhib Med Chem 34:799-807 (2019). PubMed: 30879343
  • Phua T  et al. Angiopoietin-like 4 Mediates Colonic Inflammation by Regulating Chemokine Transcript Stability via Tristetraprolin. Sci Rep 7:44351 (2017). PubMed: 28287161
  • Buler M  et al. Energy-sensing Factors Coactivator Peroxisome Proliferator-activated Receptor ? Coactivator 1-a (PGC-1a) and AMP-activated Protein Kinase Control Expression of Inflammatory Mediators in Liver: INDUCTION OF INTERLEUKIN 1 RECEPTOR ANTAGONIST. J Biol Chem 287:1847-60 (2012). PubMed: 22117073

レビューと Q&A

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1-5 of 5 Abreviews or Q&A

Question

Hola :
Yo necesito anticuerpos para Elisa (captura y detección) así como la proteína para realizar las curvas.
El cliente no desea comprar los kits, sino estos tres componentes (captura , detección y proteína) por separado.
Me podrías confirmar si para IL-1B son correctas las recomendaciones que hare o que tengo que tomar en cuenta para cotizar los productos requeridos?
**IL-1B
ab8320, ab97023, ab9617
De ser así ya busco para las otras moléculas
**il6
**il10
*tnf alpha
**ifn alpha
*ifn beta
Disculpa estaré enviando cierta cantidad de correos, pero estamos en un congreso representando a abcam y han salido varias cuestiones.
Saludos Cordiales. Muchas Gracias¡

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Answer

Gracias por contactarnos de nuevo.

Contáctanos tantas veces como sea necesario, no es ningún problema, estaré encantada de poder ayudar.

Para detectar IL1 beta por Sándwich ELISA, ab8320 es una buena opción, como puedes ver en su ficha técnica, es una aplicación testada. Pero en aquellos anticuerpos testados en sELISA, en la datasheet siempre se indicará si dicho anticuerpo actúa como captura o detección, y asimismo se indicará el anticuerpo par con el que se ha testado. En el caso de ab8320, éste se ha testado en sELISA conjuntamente con ab8321, conjugado a la enzima HRP.

Si lo que desea el cliente es hacer un ensayo de ELISA indirecto, ab8320 también puede usarse, y el secundario propuesto, al haberse usado también en ELISA, es una buena opción.

Sin embargo, la proteína sugerida no es la más adecuada para trabajar con estos anticuerpos. Como verás en su datasheet, en el apartado de Application Notes, otros primarios se sugieren para realizar un ensayo ELISA: ab18642 y ab18637.

Por tanto para el caso de IL1B yo recomendaría la proteína ab9617 con uno de los anticuerpos sugeridos, y el secundario también indicado.

Espero haberte ayudado. Si necesitas más ayuda con el resto de proteínas a detectar, no dudes en contactarme.

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Question

Here is the information requested.  

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Answer

I have processed your request to receive new vials of ab8319 and ab8321. Your new order numbers are****** and ******. Both antibodies re in stock and should arrive by 10.30am tomorrow morning.

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Question

our user recieved ab9617. lot: 715653 lot: 729507 depends on the lot number, buffer is different according to datasheet. could you please let us know how to make buffer for this antibody? Thank you so much. I look forward to hearing from you soon.

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Answer

Thank you for your enquiry. Please follow the reconstitution notes below for these 2 lots. Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml (depending on your needs). Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC. I hope this information helps. If there is anything else that I can help you with, please do not hesitate to contact me.

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Question

Can you tell me the best reconsutitution buffers for Lot. 460488?

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Answer

Thank you for your enquiry. The reconstitution notes for lot 460488 is as below: [Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.] If there is anything else that I can help you with, please do not hesitate to contact me.

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Question

How should ab9617 be reconstituted?

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Answer

Thank you for your recent phone call. The reconstitution depends on the lot of protein you received, I was able to find out that for your particular order the protein is best reconstituted in water, at a final concentration of 0.1-1mg/ml. Best of luck with your experiments.

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