Recombinant human IGFBP6 protein (Active) (ab245822)
Key features and details
- Expression system: BTI-TN-5B1-4 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human IGFBP6 protein (Active)
IGFBP6 タンパク質・ペプチド 製品一覧 -
生理活性
Determined by its ability to inhibit IGF-II induced proliferation of human MCF7 cells. The expected ED50 for this effect is 0.1 – 0.4 μg/ml.
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精製度
> 95 % SDS-PAGE.
Greater than 95% by HPLC analyses. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
BTI-TN-5B1-4 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
RCPGCGQGVQAGCPGGCVEEEDGGSPAEGCAEAEGCLRREGQECGVYTPN CAPGLQCHPPKDDEAPLRALLLGRGRCLPARAPAVAEENPKESKPQAGTA RPQDVNRRDQQRNPGTSTTPSQPNSAGVQDTEMGPCRRHLDSVLQQLQTE VYRGAQTLYVPNCDHRGFYRKRQCRSSQGQRRGPCWCVDRMGKSLPGSPD GNGSSSCPTGSSG -
予測される分子量
23 kDa -
領域
28 to 240 -
配列の追加情報
Full-length mature chain lacking the signal peptide.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab245822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
Migrates at an apparent molecular weight of approximately 23.0-30.0 kDa by SDS-PAGE analysis under non-reducing conditions.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.29% Sodium citrate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in water to 0.1 - 1.0 mg/ml.
関連情報
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別名
- IBP 6
- IBP-6
- IBP6
see all -
機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
翻訳後修飾
O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab245822 は論文での使用が確認できていません。
