Recombinant human IGFBP2 protein (ab63223)
Key features and details
- Expression system: Insect cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human IGFBP2 protein -
生理活性
Biological Activity : Determined by its ability to inhibit IGF-I induced proliferation of FDC-P1 cells.
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精製度
> 98 % SDS-PAGE.
Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is < 0.1 ng/µg of protein (<1EU/µg). -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Insect cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
EVLFRCPPCT PERLAACGPP PVAPPAAVAA VAGGARMPCA ELVREPGCGC CSVCARLEGE ACQVYTPRCG QGLRCYPHPG SELPLQALVM GEGTCEKRRD AEYGASPEQV ADNGDDHSEG GLVENHVDST MNMLGGGGSA GRKPLKSGMK ELAVFREKVT EQHRQMGKGG KHHLGLEEPK KLRPPPARTP CQQELDQVLE RISTMRLPDE RGPLEHLYSL HIPNCDKHGL YNLKQCKMSL NGQRGECWCV NPNTGKLIQG APTIRGDPEC HLFYNEQQEA RGVHTQRMQ -
予測される分子量
32 kDa -
領域
37 to 325 -
配列の追加情報
The sequence is the full length mature form minus the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab63223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成For lot specific reconstitution information please contact our Scientific Support Team.
関連情報
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別名
- BP 2
- BP2
- IBP 2
see all -
機能
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
ドメイン
The C-terminus is required for IGF-binding and growth inhibition. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
画像
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Western blot - Recombinant human IGFBP2 protein (ab63223)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab63223 は 4 報の論文で使用されています。
- Tan X et al. A protumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma. J Clin Invest 131:N/A (2021). PubMed: 32931483
- Shimizu M et al. Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression. Cancers (Basel) 13:N/A (2021). PubMed: 34572779
- Li T et al. IGFBP2 regulates PD-L1 expression by activating the EGFR-STAT3 signaling pathway in malignant melanoma. Cancer Lett 477:19-30 (2020). PubMed: 32120023
- Chua CY et al. IGFBP2 potentiates nuclear EGFR-STAT3 signaling. Oncogene 35:738-47 (2016). PubMed: 25893308
