Recombinant Human IGF2BP2/IMP-2 protein (ab173053)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus, T7 tag N-Terminus
- Suitable for: HPLC, SDS-PAGE
製品の詳細
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製品名
Recombinant Human IGF2BP2/IMP-2 protein
IGF2BP2/IMP-2 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MASMTGGQQMGRGSEFMMNKLYIGNLSPAVTADDLRQLFGDRKLPLAGQV LLKSGYAFVDYPDQNWAIRAIETLSGKVELHGKIMEVDYSVSKKLRSRKI QIRNIPPHLQWEVLDGLLAQYGTVENVEQVNTDTETAVVNVTYATREEAK IAMEKLSGHQFENYSFKISYIPDEEVSSPSPPQRAQRGDHSSREQGHAPG GTSQARQIDFPLRILVPTQFVGAIIGKEGLTIKNITLEHHHHHH -
予測される分子量
27 kDa including tags -
領域
1 to 220 -
タグ
His tag C-Terminus , T7 tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab173053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
SDS-PAGE
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製品の状態
Lyophilized -
備考
Previously labelled as IGF2BP2.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: Phosphate Buffer, 0.877% Sodium chloride
Supplied as a 0.2 µM filtered solution -
再構成Always centrifuge tubes before opening. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in ddH2O. Aliquot the reconstituted solution to minimize freeze-thaw cycles. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
関連情報
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別名
- Hepatocellular carcinoma autoantigen p62
- IF2B2_HUMAN
- IGF 2 mRNA binding protein 2
see all -
機能
Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. -
組織特異性
Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. -
配列類似性
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
ドメイン
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
細胞内局在
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab173053 は 1 報の論文で使用されています。
- Wu X et al. Long Non-Coding RNA CCAT2 Promotes the Development of Esophageal Squamous Cell Carcinoma by Inhibiting miR-200b to Upregulate the IGF2BP2/TK1 Axis. Front Oncol 11:680642 (2021). PubMed: 34386421
