Recombinant Human IGF2 protein (Active) (ab283420)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, MS, HPLC, Cell Culture, Biological Activity
製品の詳細
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製品名
Recombinant Human IGF2 protein (Active)
IGF2 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent proliferation of MCF7 cells. ED50 is ≤5.8 ng/mL, corresponding to a specific activity of 1.72 x 105 units/mg.
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精製度
>= 95 % HPLC. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
AYRPSETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRS CDLALLETYCATPAKSE -
予測される分子量
20 kDa -
領域
25 to 91 -
配列の追加情報
N-terminal glycine.
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製品の詳細
Human IGF2 protein
特性
Our Abpromise guarantee covers the use of ab283420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
HPLC
Cell Culture
Biological Activity
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- C11orf43
- IGF 2
- IGF II
see all -
機能
The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
関連疾患
Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. -
配列類似性
Belongs to the insulin family. -
翻訳後修飾
O-glycosylated with a core 1 or possibly core 8 glycan. -
細胞内局在
Secreted. - Information by UniProt
画像
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Biological Activity - Recombinant Human IGF2 protein (Active) (ab283420)
Functional analysis of ab283420.
Fully biologically active determined by the dose dependent proliferation of MCF7 cells. ED50 is ≤5.8 ng/mL, corresponding to a specific activity of 1.72 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3430138-2
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SDS-PAGE - Recombinant Human IGF2 protein (ab283420)SDS-PAGE analysis of ab283420.
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Mass Spectrometry - Recombinant Human IGF2 protein (ab283420)Mass determination by ESI-TOF.
Predicted MW is 7532.52 (+/- 10Da by ESI-TOF). MW observed 7532.89.
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HPLC - Recombinant Human IGF2 protein (ab283420)HPLC analysis of ab283420.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab283420 は論文での使用が確認できていません。
