Recombinant human IGF1 protein (Active) (ab9573)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品の詳細
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製品名
Recombinant human IGF1 protein (Active)
IGF1 タンパク質・ペプチド 製品一覧 -
生理活性
The ED50, as determined by a cell proliferation assay using FDC-P1 cells, is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.
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精製度
> 98 % SDS-PAGE.
>98% HPLC analyses. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA -
予測される分子量
8 kDa -
領域
49 to 118
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab9573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with 100 ul of deionised water (or equivalent Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
関連情報
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別名
- IBP1
- IGF I
- IGF IA
see all -
機能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
関連疾患
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
配列類似性
Belongs to the insulin family. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab9573 は 6 報の論文で使用されています。
- Zeng J et al. Brassicasterol inhibits hepatitis B virus-associated hepatocellular carcinoma development via suppression of AKT signaling pathway. Infect Agent Cancer 18:22 (2023). PubMed: 37081537
- Lebedev TD et al. The Different Impact of ERK Inhibition on Neuroblastoma, Astrocytoma, and Rhabdomyosarcoma Cell Differentiation. Acta Naturae 13:69-77 (2021). PubMed: 35127149
- Hernandez DM et al. IPF pathogenesis is dependent upon TGFß induction of IGF-1. FASEB J N/A:N/A (2020). PubMed: 32067272
- Shi Y et al. STAT3 activation regulated circ-STAT3.46 promote expression of IGF1R by sponging of miR-139-5p in human colon cancer. Transl Cancer Res 8:2593-2601 (2019). PubMed: 35117016
- De Vincenzo A et al. Paracrine recruitment and activation of fibroblasts by c-Myc expressing breast epithelial cells through the IGFs/IGF-1R axis. Int J Cancer 145:2827-2839 (2019). PubMed: 31381136
- Li N et al. The role of Zeb1 in the pathogenesis of morbidly adherent placenta. Mol Med Rep 20:2812-2822 (2019). PubMed: 31322233
