AB323023

Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard

Name: Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (ab323023) | Abcam
Brand: Abcam Limited
SKU: AB323023
Price: 29000 JPY
Availability: InStock

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Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

別名を表示する

SIDS, IDS, Iduronate 2-sulfatase, Alpha-L-iduronate sulfate sulfatase, Idursulfase

2 Images

Sandwich ELISA - Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (AB323023)

sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (AB323023)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (AB323023)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard (AB323023)

SDS-PAGE analysis of ab323023 under reducing conditions for 2ug protein.

Key facts

精製度

>80% SDS-PAGE

発現系

HEK 293 cells

アプリケーション

sELISA, SDS-PAGE

applications

生物活性

アクセッション番号

P22304

アニマルフリー

Yes

キャリアフリー

種

Human

バッファー組成

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.

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配列情報

[{"linker":null,"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"59.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P22304","tags":[]}]

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification His Tag

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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一般的な情報

機能

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.

配列の類似性

Belongs to the sulfatase family.

翻訳後修飾

Synthesized as a 75-kDa precursor form in the endoplasmic reticulum (ER), and then processed by proteolytic cleavage through various intermediates to yield a 55-kDa mature form, with the release of an 18 kDa polypeptide.. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

細胞内局在性

Lysosome

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.

See full target information IDS

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Abcam product promise

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Recombinant Human Iduronate 2 sulfatase/SIDS Protein Standard