Recombinant human ICOS protein (Fc Chimera Active) (ab245821)
Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, HPLC, SDS-PAGE
製品の詳細
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製品名
Recombinant human ICOS protein (Fc Chimera Active)
ICOS タンパク質・ペプチド 製品一覧 -
生理活性
Determined by its ability to cause adhesion of the human HL-60 monocytic cells in the presence of 2% PHA. The ED50 for this effect is 0.5-1.0 μg/ml.
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精製度
> 95 % SDS-PAGE.
Greater than 95% by HPLC analyses. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
CHO cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
EINGSANYEMFIFHNGGVQILCKYPDIVQQFKMQLLKGGQILCDLTKTKG SGNTVSIKSLKFCHSQLSNNSVSFFLYNLDHSHANYYFCNLSIFDPPPFK VTLTGGYLHIYESQLCCQLKGGPKSCDKTHTCPPCPAPELLGGPSVFLFP PKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREE QYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPR EPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTT PPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLS PGK -
予測される分子量
40 kDa including tags -
領域
21 to 140 -
タグ
Fc tag C-Terminus -
配列の追加情報
Extracellular portion of ICOS fused to the 231-amino-acid-length Fc portion of human IgG1 by two glycines.
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特性
Our Abpromise guarantee covers the use of ab245821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
HPLC
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in water to 0.1 - 1.0 mg/ml.
関連情報
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別名
- Activation inducible lymphocyte immunomediatory molecule
- Activation-inducible lymphocyte immunomediatory molecule
- AILIM
see all -
機能
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. -
組織特異性
Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus. -
関連疾患
Immunodeficiency, common variable, 1 -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab245821 は論文での使用が確認できていません。
