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AB307632

Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active)

Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active)

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Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) is a Human Fragment protein, in the 19 to 256 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC, Mass Spec, FuncS.

別名を表示する

CD275, B7H2, B7RP1, ICOSL, KIAA0653, ICOSLG, ICOS ligand, B7 homolog 2, B7-like protein Gl50, B7-related protein 1, B7-H2, B7RP-1

4 Images
Functional Studies - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) (AB307632)
  • FuncS

Supplier Data

Functional Studies - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) (AB307632)

Loaded Human ICOS Ligand, Human Fc Tag on Protein A Biosensor can bind Human ICOS, His Tag with an affinity constant of 117 nM as determined in BLI assay (GatorBio Prime).

Mass Spectrometry - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) (AB307632)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) (AB307632)

Mass determination by ESI-TOF. Predicted MW is 52358.50 (+/-10 Da by ESI-TOF). Observed MW is 52237.13 Da.

HPLC - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) (AB307632)
  • HPLC

Supplier Data

HPLC - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) (AB307632)

HPLC analysis of ab307632

SDS-PAGE - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) (AB307632)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human ICOS Ligand/ICOSL Protein (Fc Chimera) (Active) (AB307632)

SDS-PAGE analysis of ab307632

Key facts

精製度

>95% HPLC

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

Fc tag C-Terminus

アプリケーション

HPLC, FuncS, Mass Spec, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Loaded Human ICOS Ligand, Human Fc Tag on Protein A Biosensor can bind Human ICOS, His Tag with an affinity constant of 117 nM as determined in BLI assay (GatorBio Prime).

アクセッション番号

O75144

アニマルフリー

Yes

キャリアフリー

No

種

Human

再構成

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAAT","proteinLength":"Fragment","predictedMolecularWeight":"52.36 kDa","actualMolecularWeight":"52.24 kDa","aminoAcidEnd":256,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O75144","tags":[{"tag":"Fc","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
Ambient
長期保存温度
Ambient
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The ICOS Ligand also known as ICOSL B7-H2 and HK53 is a critical component in immune system function. This protein with a molecular mass of approximately 65 kDa is expressed on professional antigen-presenting cells such as dendritic cells macrophages and B cells. It plays a significant mechanical role in the immune response by engaging with its counter receptor the ICOS protein present on the surface of activated T cells. This interaction supports the regulation of T cell activity influencing proliferation and cytokine production.
Biological function summary

ICOSL contributes to the modulation of immune responses. By forming a complex with the active ICOS protein ICOSL is involved in the co-stimulation of T cells. This process is important for the development of T-cell-dependent immune responses and helps in promoting the survival and differentiation of T cells. Together the ICOS ligand and ICOS protein create a dynamic interaction that balances immune activation and tolerance which is essential for maintaining immune homeostasis.

Pathways

The ICOS-ICOSL interaction plays an important role in the T-cell receptor (TCR) signaling pathway. This pathway is fundamental for the activation of T cells and subsequent immune response. ICOSL also connects to the PI3K/Akt pathway where it impacts cell survival and proliferation. ICOS and the PI3K/Akt pathway work together to influence various aspects of immune regulation and adaptive immunity.

ICOSL has a notable impact on autoimmune diseases and cancer. Its function in immune modulation when dysregulated can contribute to autoimmune conditions such as systemic lupus erythematosus. In this context the abnormal activation of the ICOS protein may exaggerate immune responses leading to pathology. In cancer high expression of ICOSL can support tumor progression by affecting the immune surveillance capability. These associations reveal ICOSL's role in balancing immune activation and inhibition which is important in the context of disease.
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製品の性状

製品の状態

Lyophilized

補足情報

SDS-PAGE >= 95%

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一般的な情報

機能

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed : 11007762, PubMed : 11023515, PubMed : 30498080). Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP) (PubMed : 30498080).

配列の類似性

Belongs to the immunoglobulin superfamily. BTN/MOG family.

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製品プロトコール

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ターゲットの情報

Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion (PubMed : 11007762, PubMed : 11023515, PubMed : 30498080). Also induces B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity). In endothelial cells, required for proper neutrophil transmigration in response to chemoattractants, such as CXCL8/IL8 or N-formyl-methionyl peptides (fMLP) (PubMed : 30498080).
See full target information ICOSLG
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