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AB152360

Recombinant Human HuR / ELAVL1 protein (GST tag N-Terminus)

Recombinant Human HuR / ELAVL1 protein (GST tag N-Terminus)

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(1 Publication)

Recombinant Human HuR / ELAVL1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 362 aa range, expressed in Wheat germ, with >80%, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

HUR, ELAVL1, ELAV-like protein 1, Hu-antigen R, HuR

1 Images
SDS-PAGE - Recombinant Human HuR / ELAVL1 protein (GST tag N-Terminus) (AB152360)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human HuR / ELAVL1 protein (GST tag N-Terminus) (AB152360)

12.5% SDS-PAGE analysis of ab152360 stained with Coomassie Blue.

Key facts

精製度

>80% SDS-PAGE

発現系

Wheat germ

タグ

GST tag N-Terminus

アプリケーション

WB, ELISA, SDS-PAGE

applications

生物活性

No

アクセッション番号

Q15717

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Proteins & Peptides

AB51992

EGFP protein (His tag)

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Primary Antibodies

AB200342

Anti-HuR / ELAVL1 antibody [EPR17397]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody [EPR17397] (AB200342)

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  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

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Primary Antibodies

AB275876

Anti-KRAS antibody [EPR23474-76]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-KRAS antibody [EPR23474-76] (AB275876)

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  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK","proteinLength":"Full Length","predictedMolecularWeight":"62.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":362,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q15717","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HuR also known as ELAVL1 is a protein that binds RNA in cells. It weighs about 36 kDa and is found in the nucleus and cytoplasm. Its name HuR comes from its similarity to human antigen R. Researchers often call it the HuR protein or ELAVL1 protein. This protein helps stabilize certain mRNA molecules by binding to AU-rich elements (AREs) in their 3' untranslated regions. Its binding affects mRNA decay rates and therefore influences protein synthesis.
Biological function summary

HuR influences cell growth differentiation and stress responses. It does not work alone; it often forms part of larger ribonucleoprotein complexes. By stabilizing mRNAs HuR ensures the continuous production of proteins necessary for cell survival and adaptation to stressors. This regulatory role highlights its involvement in many cellular processes making it a focus for study in relation to cellular homeostasis.

Pathways

HuR is part of the mRNA surveillance pathway and the response to cellular stress. HuR modulates gene expression by interacting with proteins involved in transcription and translation regulation. It frequently partners with proteins like TTP (tristetraprolin) that also bind to AREs affecting mRNA stability and translation processes. Such interactions extend HuR's influence across pathways related to gene expression regulation and response to stress.

Researchers associate HuR with cancer and neurodegenerative diseases. In cancer HuR stabilizes mRNAs of many oncogenes aiding tumor growth and progression. It may interact with proteins like p53 affecting tumor suppressor pathways. For neurodegenerative diseases HuR's role in mRNA stability can influence neural health connected to proteins like TDP-43 in conditions such as Alzheimer's and ALS. Understanding HuR's function offers insights into potential therapeutic targets for these diseases.
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一般的な情報

機能

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed : 14517288, PubMed : 18285462, PubMed : 31358969). Involved in embryonic stem cell (ESC) differentiation : preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed : 32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398, PubMed : 17632515, PubMed : 18285462, PubMed : 23519412, PubMed : 8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed : 8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed : 29180010).

配列の類似性

Belongs to the RRM elav family.

翻訳後修飾

Phosphorylated by MAPKAPK2 (PubMed:14517288). Phosphorylated by PRKCD (PubMed:18285462).. Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.

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製品プロトコール

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ターゲットの情報

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed : 14517288, PubMed : 18285462, PubMed : 31358969). Involved in embryonic stem cell (ESC) differentiation : preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed : 32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398, PubMed : 17632515, PubMed : 18285462, PubMed : 23519412, PubMed : 8626503). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed : 8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed : 29180010).
See full target information ELAVL1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 62:101144 PubMed39987653

2025

m6A eraser ALKBH5/treRNA1/DDX46 axis regulates BCR expression.

Applications

Unspecified application

Species

Unspecified reactive species

Bandish Kapadia,Anirban Roychowdhury,Forum Kayastha,Won Sok Lee,Nahid Nanaji,Jolene Windle,Ronald Gartenhaus
View all publications
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