AB217833

Recombinant Human HtrA2 / Omi protein (His tag)

Name: Recombinant Human HtrA2 / Omi protein (His tag) (ab217833) | Abcam
Brand: Abcam Limited
SKU: AB217833
Price: 75000 JPY
Availability: InStock

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Recombinant Human HtrA2 / Omi protein (His tag) is a Human Fragment protein, in the 133 to 358 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

別名を表示する

OMI, PRSS25, HTRA2, High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HtrA2

1 Images

SDS-PAGE - Recombinant Human HtrA2 / Omi protein (His tag) (AB217833)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human HtrA2 / Omi protein (His tag) (AB217833)

SDS-PAGE analysis of ab217833.

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

O43464

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7 Preservative: 1.02% Imidazole Constituents: 10% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.79% Tris HCl

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"AAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQ","proteinLength":"Fragment","predictedMolecularWeight":"29 kDa","actualMolecularWeight":null,"aminoAcidEnd":358,"aminoAcidStart":133,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43464","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HtrA2 also known as Omi or the Omi protein is a serine protease with a molecular mass of approximately 50 kDa. It is a mitochondrial protein and commonly found in the cytosol following cellular stress. HtrA2/Omi mediates its function through its protease and pro-apoptotic activities. It is involved in the degradation of damaged proteins and exerts an important role in maintaining protein quality control within mitochondria. Researchers often study HtrA2/Omi for its function in apoptosis and Parkinson's disease-related pathways.

Biological function summary

The degradation and signaling processes of HtrA2/Omi protect against proteotoxic stress. It operates within the mitochondria and under stress conditions relocates to the cytosol where it can influence apoptosis. HtrA2/Omi can interact with inhibitors of apoptosis proteins (IAPs) and by doing this promotes apoptosis when necessary. This regulation of cell death links HtrA2/Omi to the cellular quality control mechanisms directly impacting cell survival and health.

Pathways

HtrA2/Omi integrates into the mitochondrial apoptosis pathway and is important in Parkinson's disease pathways. Through its interactions with apoptosis regulators like XIAP HtrA2/Omi facilitates the removal of unwanted cells. Additionally it participates in signaling pathways involving caspases and other pro-apoptotic factors. Its activity ensures proper communication and response within these pathways to maintain cellular balance and respond to stressors or damage.

HtrA2/Omi links to neurodegenerative conditions such as Parkinson's disease and Huntington's disease. Mutations or alterations in expression levels can contribute to these conditions by disrupting normal apoptotic signaling. Other proteins like PINK1 and Parkin are closely related to HtrA2/Omi in the context of Parkinson's disease where they collectively regulate mitochondrial function and health. In such disorders the malfunction of HtrA2/Omi or its interactions leads to impaired cell survival and increased susceptibility to neuronal damage.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Isoform 1. Serine protease that shows proteolytic activity against a non-specific substrate beta-casein (PubMed : 10873535). Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism (PubMed : 15200957). Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6 (PubMed : 36758104, PubMed : 36758105). Cleaves THAP5 and promotes its degradation during apoptosis (PubMed : 19502560).. Isoform 2. Seems to be proteolytically inactive.

配列の類似性

Belongs to the peptidase S1C family.

翻訳後修飾

Ubiquitinated by BIRC6; this activity is inhibited by DIABLO/SMAC.. Autoproteolytically activated.

細胞内局在性

Mitochondrion intermembrane space

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information HTRA2

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Abcam product promise

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Recombinant Human HtrA2 / Omi protein (His tag)