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Recombinant human Hsp70 protein (ab80031)

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SDS-PAGE - Recombinant human Hsp70 protein (ab80031)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 90% SDS-PAGE
    • Active: Yes
    • Suitable for: WB, ELISA, Functional Studies, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant human Hsp70 protein
      Hsp70 タンパク質・ペプチド 製品一覧

    • 生理活性

      ab80031 tested positive for ATPase activity using a Malachite Green assay.

    • 精製度

      > 90 % SDS-PAGE.
      ab80031 is Multi-step chromatographically purified.

    • 発現系

      Baculovirus infected Sf9 cells

    • アクセッション番号

      P0DMV8
      P0DMV9

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • アミノ酸配列 1

      • 生物種

        Human

      • 配列

        MAKAAAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERL IGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDK PKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYF NDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDL GGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHK KDISQNKRAVRRLRTACERAKRTLSSSTGASLEIDSLFEGIDFYTSITRA RFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLL QDFFNGRDLNKSINPDEAVGYGAAVQAAILMGDKSENVQDLLLLDVAPLS LGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMT KDNNLLGRFELSCIPPAPGVPQIEVTFDIDANGILNVTATKDSTGKANKI TITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKS AVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQ VCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD

      • アミノ酸配列 2

      • 生物種

        Human

      • 予測される分子量

        70 kDa

    特性

    Our Abpromise guarantee covers the use of ab80031 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      ELISA

      Functional Studies

      SDS-PAGE

    • 製品の状態

      Liquid

    • 備考

      ab80031 is endotoxin free.
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

      Constituents: 0.79% Tris HCl, 0.00292% EDTA, 10% Glycerol (glycerin, glycerine), 1.74% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    関連情報

    • 別名

      • DnaK type molecular chaperone HSP70 1
      • Epididymis secretory protein Li 103
      • FLJ54303
      • FLJ54370
      • FLJ54392
      • FLJ54408
      • FLJ75127
      • Heat shock 70 kDa protein 1
      • Heat shock 70 kDa protein 1/2
      • Heat shock 70 kDa protein 1A
      • Heat shock 70 kDa protein 1A/1B
      • Heat shock 70kDa protein 1B
      • Heat shock induced protein
      • HEL S 103
      • HSP70 1
      • HSP70 1A
      • HSP70 1B
      • HSP70 2
      • HSP70-1
      • HSP70-1/HSP70-2
      • HSP70-1A
      • HSP70-2
      • HSP70.1
      • HSP70.1/HSP70.2
      • HSP71_HUMAN
      • HSP72
      • HSPA1
      • HSPA1A
      • HSPA1B
      see all

    • 関連性

      Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific.

    • 細胞内局在

      Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

    画像

    • SDS-PAGE - Recombinant human Hsp70 protein (ab80031)
      SDS-PAGE - Recombinant human Hsp70 protein (ab80031)

      Lane 1. Molecular weight marker

      Lane 2. Hsp70 protein

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab80031 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab80031 は論文での使用が確認できていません。

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