Recombinant human Hsc70 protein (ab78431)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Affinity purified
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: WB, ELISA, Functional Studies, Competitive Binding Assays, SDS-PAGE
製品の詳細
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製品名
Recombinant human Hsc70 protein
Hsc70 タンパク質・ペプチド 製品一覧 -
生理活性
ab78431 has ATPase activity at the time of manufacture of 3.2 µM phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1 mM ATP using a Malachite Green assay.
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精製度
> 90 % Affinity purified.
ab78431 is affinity purified. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
73 kDa including tags -
領域
1 to 646 -
タグ
His tag N-Terminus -
配列の追加情報
His tag
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab78431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
ELISA
Functional Studies
Competitive Binding Assays
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
pH: 8
Constituents: 1.753% Sodium chloride, 0.788% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 2410008N15Rik
- Constitutive heat shock protein 70
- Epididymis luminal protein 33
see all -
機能
Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the heat shock protein 70 family. -
ドメイン
The N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
細胞内局在
Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
画像
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SDS-PAGE - Recombinant human Hsc70 protein (ab78431)SDS-PAGE of 73kDa Hsc70 protein.
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SDS-PAGE - Recombinant human Hsc70 protein (ab78431)Lane 1. Molecular weight ladder
Lane 2. Hsc70 protein
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab78431 は 6 報の論文で使用されています。
- Zhang KK et al. LAMP2A, and other chaperone-mediated autophagy related proteins, do not decline with age in genetically heterogeneous UM-HET3 mice. Aging (Albany NY) 15:4685-4698 (2023). PubMed: 37315291
- Endicott SJ et al. Long-lived mice with reduced growth hormone signaling have a constitutive upregulation of hepatic chaperone-mediated autophagy. Autophagy 17:612-625 (2021). PubMed: 32013718
- Xu X et al. Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model. Protein Cell 12:769-787 (2021). PubMed: 34291435
- Fan Y et al. Acetylation-dependent regulation of TPD52 isoform 1 modulates chaperone-mediated autophagy in prostate cancer. Autophagy 17:4386-4400 (2021). PubMed: 34034634
- Endicott SJ et al. Inhibition of class I PI3K enhances chaperone-mediated autophagy. J Cell Biol 219:N/A (2020). PubMed: 33048163
- Labrador-Garrido A et al. Chaperome screening leads to identification of Grp94/Gp96 and FKBP4/52 as modulators of the a-synuclein-elicited immune response. FASEB J 30:564-77 (2016). PubMed: 26443817
