Recombinant human HRG beta 1 protein (ab187226)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human HRG beta 1 protein
HRG beta 1 タンパク質・ペプチド 製品一覧 -
生理活性
The activity of HRG beta 1 is determined by the ability to stimulate proliferation of MC7 cells under serum free conditions and is typically less than 0.3 ng/mL.
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精製度
> 95 % SDS-PAGE.
The purity of ab187226 is determined by reducing and non-reducing SDS-PAGE. -
エンドトキシン・レベル
<=1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSHLVKCAEK EKTFCVNGGE CFMVKDLSNP SRYLCKCPNE FTGDRCQNYV MASFYKHLGI EFMEAE -
予測される分子量
8 kDa -
領域
177 to 241 -
配列の追加情報
The protein sequence corresponds to isoform 6 of human HRG beta 1 protein.
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特性
Our Abpromise guarantee covers the use of ab187226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C long term. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
関連情報
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別名
- Acetylcholine receptor inducing activity
- ARIA
- Breast cancer cell differentiation factor p45
see all -
関連性
HRG beta 1 is an isoform from the Neuregulin/Heregulin family of structurally related polypeptide growth factors which are derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. HRG1-ß1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. Although HRG1-ß1 biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility promoting factor (AMF). -
細胞内局在
Secreted
画像
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SDS-PAGE - Recombinant human HRG beta 1 protein (ab187226)
SDS PAGE analysis of ab187226 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab187226 は論文での使用が確認できていません。
