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AB60867

Recombinant ヒト HIPK1 protein (GST tag N-Terminus)

Recombinant human HIPK1 protein (GST tag N-Terminus)

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Recombinant human HIPK1 protein (GST tag N-Terminus) is a Human Fragment protein, in the 156 to 555 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

KIAA0630, MYAK, NBAK2, HIPK1, Homeodomain-interacting protein kinase 1, Nuclear body-associated kinase 2

5 Images
Functional Studies - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)
  • FuncS

Unknown

Functional Studies - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)

Sample Kinase Activity Plot.

Functional Studies - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)
  • FuncS

Unknown

Functional Studies - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)

The specific activity of HIPK1 (ab60867) was determined to be 79 nmol/min/mg as per activity assay protocol

Functional Studies - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)
  • FuncS

Unknown

Functional Studies - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)

Sample Kinase Activity Plot.

SDS-PAGE - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)

ab60867 on SDS-PAGE, MW ~71 kDa.

SDS-PAGE - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human HIPK1 protein (GST tag N-Terminus) (AB60867)

SDS PAGE analysis of ab60867

Key facts

精製度

>90% Densitometry

Affinity purified.

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Active

アクセッション番号

Q86Z02

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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組合せ製品

Proteins & Peptides

AB78694

Recombinant Human Calmodulin 1/2/3 protein (Tag Free)

SDS-PAGE - Recombinant Human Calmodulin 1/2/3 protein (Tag Free) (AB78694)

  • 0
  • 0
Proteins & Peptides

AB131707

Recombinant human CASK protein (GST tag N-Terminus)

Functional Studies - Recombinant human CASK protein (GST tag N-Terminus) (AB131707)

  • 0
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Proteins & Peptides

AB85758

Recombinant human DCLK2 protein (GST tag N-Terminus)

Functional Studies - Recombinant human DCLK2 protein (GST tag N-Terminus) (AB85758)

  • 0
  • 0
Primary Antibodies

AB259945

Anti-PDE5A/PDE5 antibody [EPR24129-95]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE5A/PDE5 antibody [EPR24129-95] (AB259945)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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配列情報

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":555,"aminoAcidStart":156,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q86Z02","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HIPK1 also known as Homeodomain-Interacting Protein Kinase 1 functions as a serine/threonine kinase. It has a molecular mass of approximately 132 kDa. This protein acts in the regulation of transcription factors and apoptosis. HIPK1 is widely expressed with higher levels in the brain heart and lung tissues. Researchers sometimes investigate its roles in several signal transduction pathways due to its regulatory functions.
Biological function summary

HIPK1 participates in various cellular processes including apoptosis DNA damage response and Wnt signaling. It forms complexes with several transcription factors such as p53 and Smad proteins modifying their activity to influence cell fate. By interacting with transcription regulators HIPK1 modulates gene expression and has implications in cell growth and differentiation.

Pathways

HIPK1 interacts with proteins in important signaling cascades including the Wnt and JNK pathways. Notably HIPK1 phosphorylates components like c-Jun influencing the JNK pathway's role in stress response and apoptosis. Additionally in the Wnt pathway HIPK1 modulates β-catenin signaling which is important for regulating cell proliferation and differentiation. These interactions connect HIPK1 to wider cellular communications and responses.

Researchers have linked HIPK1 to cancer and fibrotic diseases. Abnormal HIPK1 expression or activity can influence oncogenic processes due to its regulatory role on p53 a protein important for tumor suppression. Moreover in fibrosis altered HIPK1 activity impacts the TGF-β pathway by interacting with Smad proteins which promotes fibrogenesis. Understanding these connections could facilitate targeting HIPK1 in therapeutic strategies for these conditions.
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一般的な情報

機能

Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. Phosphorylates PAGE4 at 'Thr-51' which is critical for the ability of PAGE4 to potentiate the transcriptional activator activity of JUN (PubMed : 24559171).

配列の類似性

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.

翻訳後修飾

Autophosphorylated. Phosphorylated and activated by JNK1.. Degraded by PARK7 at the protein level.. Sumoylated. When conjugated it is directed to nuclear speckles. SENP1-mediated desumoylation is mediated by TNF in response to stress stimuli, triggering transient translocation from nucleus to cytoplasm.

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製品プロトコール

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ターゲットの情報

Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. Phosphorylates PAGE4 at 'Thr-51' which is critical for the ability of PAGE4 to potentiate the transcriptional activator activity of JUN (PubMed : 24559171).
See full target information HIPK1
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