JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB80350

Recombinant human HDAC9 protein

Recombinant human HDAC9 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human HDAC9 protein is a Human Fragment protein, in the 604 to 1066 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

別名を表示する

HDAC7, HDAC7B, HDRP, KIAA0744, MITR, HDAC9, Histone deacetylase 9, HD9, Histone deacetylase 7B, Histone deacetylase-related protein, MEF2-interacting transcription repressor MITR, HD7, HD7b

2 Images
Functional Studies - Recombinant human HDAC9 protein (AB80350)
  • FuncS

Unknown

Functional Studies - Recombinant human HDAC9 protein (AB80350)

Image showing specific activity of ab80350.

SDS-PAGE - Recombinant human HDAC9 protein (AB80350)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human HDAC9 protein (AB80350)

10% SDS-PAGE showing ab80350 at approximately 50.7kDa (3μg).

Key facts

精製度

>95% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

タグ

Tag free

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Specific Activity: >1000 U/ug. One U =1pmol/min. Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate, and 0.2 ng/ul HDAC9. Incubation condition: 30 min at 37°C.

アクセッション番号

Q9UKV0

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.8004% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

配列情報

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":1066,"aminoAcidStart":604,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UKV0","tags":[]}]
style
left-column

出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HDAC9 also known as Histone Deacetylase 9 is a protein involved in removing acetyl groups from histone proteins leading to chromatin condensation and transcriptional repression. HDAC9 has a molecular weight of about 127 kDa. It expresses predominantly in the brain muscle tissue and immune cells. This protein belongs to the class IIa family of histone deacetylases and has close structural similarities to other class IIa HDACs like HDAC4 HDAC5 and HDAC7.
Biological function summary

HDAC9 plays a significant role in regulating gene expression by altering the acetylation status of histones. It participates in the formation of large multiprotein complexes that target specific gene promoters. HDAC9's activity impacts various cellular processes including cell cycle progression and differentiation. It is a part of the CoREST complex which interacts with REST corepressor 1 to modulate neuronal gene expression and synaptic plasticity.

Pathways

HDAC9 is actively involved in key cellular signaling cascades. It contributes to the Notch signaling pathway which has a profound impact on cell differentiation and fate decisions. It also plays a role in the NF-kB pathway which is critical for immune response and inflammation. Through these pathways HDAC9 interacts with proteins such as RBP-Jk in the Notch pathway and IKK complex members in the NF-kB pathway indicating its broad influence on cellular mechanisms.

HDAC9 associates with several pathological conditions. It has been linked to cardiovascular diseases such as cardiac hypertrophy and heart failure due to its influence on cardiac muscle gene expression. HDAC9 also relates to various cancers including colon cancer where its dysregulation affects tumorigenesis and cell proliferation. In these contexts HDAC9 interacts with proteins like MEF2 and p300 which are pivotal in cardiac growth and cancer progression respectively.
style
left-column

製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

style
left-column

一般的な情報

機能

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.

配列の類似性

Belongs to the histone deacetylase family. HD type 2 subfamily.

翻訳後修飾

Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.. Sumoylated.

細胞内局在性

Nucleus

style
left-column

製品プロトコール

style
left-column

ターゲットの情報

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
See full target information HDAC9
style
left-column
style
left-column
style
right-column

組合せ製品

Primary Antibodies

AB109446

Anti-HDAC9 antibody [EPR5223]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] (AB109446)

  • 5
  • 2
Proteins & Peptides

AB104121

Recombinant human HDAC1 protein

SDS-PAGE - Recombinant human HDAC1 protein (AB104121)

  • 0
  • 0
Proteins & Peptides

AB95246

Recombinant Human SIRT7 protein

SDS-PAGE - Recombinant Human SIRT7 protein (AB95246)

  • 0
  • 0
Proteins & Peptides

AB42630

Recombinant human HDAC2 protein

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com