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AB104029

Recombinant ヒト HDAC4 protein (GST tag N-Terminus)

Recombinant human HDAC4 protein (GST tag N-Terminus)

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(2 Publications)

Recombinant human HDAC4 protein (GST tag N-Terminus) is a Human Fragment protein, in the 612 to 1084 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

別名を表示する

KIAA0288, HDAC4, Histone deacetylase 4, HD4

2 Images
Functional Studies - Recombinant human HDAC4 protein (GST tag N-Terminus) (AB104029)
  • FuncS

Unknown

Functional Studies - Recombinant human HDAC4 protein (GST tag N-Terminus) (AB104029)

Kinase Assay demonstrating specific activity of ab104029.

SDS-PAGE - Recombinant human HDAC4 protein (GST tag N-Terminus) (AB104029)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human HDAC4 protein (GST tag N-Terminus) (AB104029)

SDS-PAGE showing ab104029 at approximately 77kDa.

Key facts

精製度

>95% SDS-PAGE

Purity was determined to be >95% by densitometry. Affinity purified.

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

The Specific activity of ab104029 was determined to be 60 RLU/min/ng.

アクセッション番号

P56524

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Proteins & Peptides

AB101661

Recombinant human HDAC1 protein (GST tag C-Terminus)

Functional Studies - Recombinant human HDAC1 protein (GST tag C-Terminus) (AB101661)

  • 0
  • 0
Proteins & Peptides

AB101660

Recombinant human HDAC7 protein (GST tag N-Terminus)

Functional Studies - Recombinant human HDAC7 protein (GST tag N-Terminus) (AB101660)

  • 0
  • 0
Proteins & Peptides

AB42632

Recombinant human HDAC6 protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human HDAC6 protein (Active) (GST tag N-Terminus) (AB42632)

  • 0
  • 0
Assay Kits

AB300311

Human HDAC4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human HDAC4 ELISA Kit (AB300311)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"77 kDa","actualMolecularWeight":null,"aminoAcidEnd":1084,"aminoAcidStart":612,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P56524","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

HDAC4 also known as Histone Deacetylase 4 functions in the regulation of gene expression through chromatin remodeling. This protein removes acetyl groups from histone proteins causing chromatin compaction and transcriptional repression. HDAC4 has a molecular weight of approximately 140 kDa. The protein is found in various tissues including brain heart and skeletal muscle and plays essential roles in various cellular processes. Alternate names for HDAC4 include KIAA0288 and HD4.
Biological function summary

The role of HDAC4 extends beyond chromatin remodeling as it serves as a critical regulator in several cellular functions. As part of a complex HDAC4 interacts with other proteins to control cell cycle differentiation and apoptosis. It cooperates with transcriptional repressors like MEF2 and RUNX2 influencing various signaling pathways. The protein's activity impacts neuronal survival muscle development and cardiac hypertrophy through its regulatory mechanisms.

Pathways

HDAC4 integrates into significant signaling networks notably the MAPK and calcium-calmodulin signaling pathways. Within the MAPK pathway HDAC4 associates with proteins like MEF2 impacting cellular growth and differentiation processes. In the calcium-calmodulin pathway HDAC4 affects gene transcription via its interaction with the phosphatase calcineurin linking intracellular calcium levels with transcriptional responses.

HDAC4 has associations with neurological disorders and cancer. Mutations and dysregulation in HDAC4 are linked to neuronal disorders such as Huntington's disease. In cancer HDAC4 interacts with other oncogenic proteins such as p53 influencing tumor progression and resistance to therapy. Consequently HDAC4 presents as a potential target for therapeutic intervention in these disease contexts.
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一般的な情報

機能

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).

配列の類似性

Belongs to the histone deacetylase family. HD type 2 subfamily.

翻訳後修飾

Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1 (PubMed : 27708256).
See full target information HDAC4
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Prostate 83:1470-1493 PubMed37559436

2023

Third generation quinoline-3-carboxamide transcriptional disrupter of HDAC4, HIF-1α, and MEF-2 signaling for metastatic castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

John T Isaacs,Susan L Dalrymple,Lizamma Antony,David Marc Rosen,Ilsa M Coleman,Peter S Nelson,Maya Kostova,Iain A Murray,Gary H Perdew,Samuel R Denmeade,Emmanuel S Akinboye,W Nathaniel Brennen

Journal of molecular cell biology 11:158-169 PubMed29800227

2018

Host HDAC4 regulates the antiviral response by inhibiting the phosphorylation of IRF3.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Yang,Jielin Tang,Rongjuan Pei,XiaoXiao Gao,Jing Guo,Chonghui Xu,Yun Wang,Qian Wang,Chunchen Wu,Yuan Zhou,Xue Hu,He Zhao,Yanyi Wang,Xinwen Chen,Jizheng Chen
View all publications
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