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AB314619

Recombinant Human GPR15 Protein (His Tag)

Recombinant Human GPR15 Protein (His Tag)

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Recombinant Human GPR15 Protein (His Tag) is a Human Full Length protein, in the 1 to 360 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

別名を表示する

G-protein coupled receptor 15, Brother of Bonzo, BoB, GPR15

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SDS-PAGE - Recombinant Human GPR15 Protein (His Tag) (AB314619)
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Supplier Data

SDS-PAGE - Recombinant Human GPR15 Protein (His Tag) (AB314619)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. It is speculated that the protein forms a dimeric structure. Observed band size : Monomer：42 kDa Dimer：84 kDa

Key facts

精製度

>85% SDS-PAGE

発現系

Escherichia coli

タグ

10x His tag N-Terminus

アプリケーション

SDS-PAGE

applications

生物活性

No

アクセッション番号

P49685

アニマルフリー

Yes

キャリアフリー

No

種

Human

再構成

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

バッファー組成

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate

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組合せ製品

Primary Antibodies

AB8104

Anti-GPCR GPR15 antibody

Western blot - Anti-GPCR GPR15 antibody (AB8104)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MDPEETSVYLDYYYATSPNSDIRETHSHVPYTSVFLPVFYTAVFLTGVLGNLVLMGALHFKPGSRRLIDIFIINLAASDFIFLVTLPLWVDKEASLGLWRTGSFLCKGSSYMISVNMHCSVLLLTCMSVDRYLAIVWPVVSRKFRRTDCAYVVCASIWFISCLLGLPTLLSRELTLIDDKPYCAEKKATPIKLIWSLVALIFTFFVPLLSIVTCYCCIARKLCAHYQQSGKHNKKLKKSIKIIFIVVAAFLVSWLPFNTFKFLAIVSGLRQEHYLPSAILQLGMEVSGPLAFANSCVNPFIYYIFDSYIRRAIVHCLCPCLKNYDFGSSTETSDSHLTKALSTFIHAEDFARRRKRSVSL","proteinLength":"Full Length","predictedMolecularWeight":"43.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":360,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P49685","tags":[{"tag":"10x His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Lyophilized
精製方法
Ion exchange chromatography
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-80°C
分注に関する情報
Add glycerol to a final volume of 50% for extra stability and aliquot
保管に関する情報
The product can be stored for up to 12 months
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GPR15 also known as G protein-coupled receptor 15 is a receptor involved in cell signaling. It has a molecular mass of approximately 41 kDa. This receptor is expressed mainly in intestinal tissues but also appears in a variety of immune cells including certain T-cells. GPR15 participates in chemokine signaling processes by binding extracellular ligands and mediating signals inside the cell through interaction with G proteins which then trigger downstream pathways.
Biological function summary

G protein-coupled receptor 15 regulates immune responses and contributes to maintaining the integrity of the intestinal epithelial barrier. It serves as a homing receptor guiding the migration of T-cells to specific tissues especially in the gut. This function is critical for both gut immune homeostasis and inflammatory responses. Although part of the larger G protein-coupled receptor complex family it operates mainly as an individual entity orchestrating responses to environmental signals.

Pathways

Signals initiated by G protein-coupled receptor 15 affect the MAPK/ERK pathway which plays a pivotal role in cell division differentiation and survival. These pathways connect GPR15 functionally to various proteins such as Ras and Raf which are also central to the path regulation. Additionally this receptor is involved in the modulation of cytokine signaling pathways influencing immune and inflammatory responses through interaction with cytokines and chemokines in its vicinity.

G protein-coupled receptor 15 has relevance to inflammatory bowel disease and HIV infection. Its involvement in immune cell homing to the gut links it to the pathology of inflammatory bowel diseases where it may exacerbate inflammation by recruiting effector T-cells. In HIV infection the virus exploits GPR15 alongside the protein CCR5 to enter target cells making this receptor a potential target for therapeutic intervention in managing the infection. Understanding GPR15's roles improves insight into both immune-related disorders and infectious disease pathways.
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一般的な情報

機能

G protein-coupled receptor that plays an important role in immune homeostasis (PubMed : 33758080, PubMed : 38918398). Acts via its natural ligand GPR15LG, a chemokine-like polypeptide strongly expressed in gastrointestinal tissues. GPR15-GPR15LG signaling axis regulates intestinal homeostasis and inflammation through the migration of immune cells (PubMed : 33758080, PubMed : 38918398). Controls thereby the specific homing of T-cells, particularly FOXP3+ regulatory T-cells (Tregs), to the large intestine lamina propria (By similarity). Also required for skin localization of thymus-derived dendritic epidermal T-cells (By similarity). Plays an important role in mediating cytoprotective function as well as angiogenesis of thrombomodulin (By similarity). Mechanistically, preferentially signals through the Gi/o pathway to inhibit adenylate cyclase activity and activate a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores (PubMed : 35510660).. (Microbial infection) Acts as an alternative coreceptor with CD4 for HIV-1 infection.

配列の類似性

Belongs to the G-protein coupled receptor 1 family.

翻訳後修飾

Phosphorylation is necessary for YWHAE binding and efficient surface expression.. O-glycosylated (PubMed:21189250). Sialylated O-glycans in the N-terminal tail inhibits binding of GPR15LG (PubMed:33758080).. Sulfation is required for efficient binding of GPR15LG.

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製品プロトコール

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ターゲットの情報

G protein-coupled receptor that plays an important role in immune homeostasis (PubMed : 33758080, PubMed : 38918398). Acts via its natural ligand GPR15LG, a chemokine-like polypeptide strongly expressed in gastrointestinal tissues. GPR15-GPR15LG signaling axis regulates intestinal homeostasis and inflammation through the migration of immune cells (PubMed : 33758080, PubMed : 38918398). Controls thereby the specific homing of T-cells, particularly FOXP3+ regulatory T-cells (Tregs), to the large intestine lamina propria (By similarity). Also required for skin localization of thymus-derived dendritic epidermal T-cells (By similarity). Plays an important role in mediating cytoprotective function as well as angiogenesis of thrombomodulin (By similarity). Mechanistically, preferentially signals through the Gi/o pathway to inhibit adenylate cyclase activity and activate a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores (PubMed : 35510660).. (Microbial infection) Acts as an alternative coreceptor with CD4 for HIV-1 infection.
See full target information GPR15
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