AB202204

Recombinant Human GORASP2/GRASP55 protein (His tag N-Terminus)

Name: Recombinant Human GORASP2/GRASP55 protein (ab202204) | Abcam
Brand: Abcam Limited
SKU: AB202204
Price: 123000 JPY
Availability: InStock

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Recombinant Human GORASP2/GRASP55 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 452 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

GOLPH6, GORASP2, Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, Golgi reassembly-stacking protein of 55 kDa, p59, GRASP55

1 Images

SDS-PAGE - Recombinant Human GORASP2/GRASP55 protein (His tag N-Terminus) (AB202204)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GORASP2/GRASP55 protein (His tag N-Terminus) (AB202204)

15% SDS-PAGE analysis of ab202204 (3 μg)

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

アクセッション番号

Q9H8Y8

アニマルフリー

キャリアフリー

Yes

種

Human

バッファー組成

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Protein previously labeled as GORASP2.

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMGSSQSVEIPGGGTEGYHVLRVQENSPGHRAGLEPFFDFIVSINGSRLNKDNDTLKDLLKANVEKPVKMLIYSSKTLELRETSVTPSNLWGGQGLLGVSIRFCSFDGANENVWHVLEVESNSPAALAGLRPHSDYIIGADTVMNESEDLFSLIETHEAKPLKLYVYNTDTDNCREVIITPNSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKISLPGQMAGTPITPLKDGFTEVQLSSVNPPSLSPPGTTGIEQSLTGLSISSTPPAVSSVLSTGVPTVPLLPPQVNQSLTSVPPMNPATTLPGLMPLPAGLPNLPNLNLNLPAPHIMPGVGLPELVNPGLPPLPSMPPRNLPGIAPLPLPSEFLPSFPLVPESSSAASSGELLSSLPPTSNAPSDPATTTAKADAASSLTVDVTPPTAKAPTTVEDRVGDSTPVSEKPVSAAVDANASESP","proteinLength":"Full Length","predictedMolecularWeight":"49.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":452,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H8Y8","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

GORASP2 also known as GRASP55 is a golgi reassembly stacking protein with a mass approximately 55 kDa. It is highly expressed in the Golgi apparatus where it plays an important role in maintaining the structure and function of this organelle. GRASP55 facilitates the stacking of Golgi cisternae which is essential for Golgi apparatus organization. The protein acts alongside its isoform GRASP65 though they function somewhat independently in different Golgi regions.

Biological function summary

GRASP55 controls various cellular processes including the trafficking of proteins and lipids within the cell. It is part of the GRASP complex along with GRASP65 facilitating membrane tethering and fusion events. The protein's function is also linked to its ability to regulate Golgi's response to cellular stress or changes in environment impacting secretion and growth factor availability. In some conditions GRASP55 can influence processes such as autophagy underlining its diverse biological actions within the cell.

Pathways

GRASP55 integrates into significant cellular pathways like the secretory pathway and the autophagy pathway. It interacts with key proteins like golgins and Rab GTPases to assist in vesicle tethering and transport. GRASP55 also closely interacts with p62 a protein that plays a critical role in the selective autophagy pathway. This positions GRASP55 as a contributor to not only the routine processing of cellular materials but also cellular adaptation to stress through autophagy modulation.

GRASP55 shows relevance to cancer progression and neurodegenerative diseases. Aberrant expression or dysfunction of GRASP55 can contribute to cancerous transformations by altering cell adhesion and signaling pathways. Its role in autophagy connects it to diseases like Alzheimer's where defective protein processing is a hallmark. The interaction of GRASP55 with proteins such as p62 becomes particularly important as disrupted autophagy pathways are often implicated in neurodegenerative conditions highlighting GRASP55's significant role in both health and disease.

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製品の性状

製品の状態

Liquid

補足情報

ab202204 was purified using conventional chromatography.

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一般的な情報

機能

Key structural protein of the Golgi apparatus (PubMed : 33301566). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (PubMed : 33301566). Acting in concert with GORASP1/GRASP65, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (PubMed : 33301566). However, other studies suggest that GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis (PubMed : 10487747, PubMed : 21515684, PubMed : 22523075). May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA (PubMed : 11101516). Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells (By similarity). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed : 21884936, PubMed : 27062250, PubMed : 28067262).

配列の類似性

Belongs to the GORASP family.

翻訳後修飾

Myristoylated (PubMed:11101516). Myristoylation is essential for the Golgi targeting (By similarity).. Palmitoylated.. Phosphorylated in mitotic cells (PubMed:11408587). ER stress-induced phosphorylation at Ser-441 induces monomerization and subsequent relocalization from Golgi to ER which is essential for mediating unconventional (ER/Golgi-independent) trafficking of CFTR to the cell membrane (PubMed:21884936, PubMed:27062250).

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information GORASP2

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Recombinant Human GORASP2/GRASP55 protein (His tag N-Terminus)